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实验室小鼠进行尾部活检时应该麻醉吗?

Should laboratory mice be anaesthetized for tail biopsy?

作者信息

Arras Margarete, Rettich Andreas, Seifert Burkhardt, Käsermann Hans Peter, Rülicke Thomas

机构信息

Institute of Laboratory Animal Science, University of Zurich, Sternwartstrasse 6, CH-8091 Zurich, Switzerland.

出版信息

Lab Anim. 2007 Jan;41(1):30-45. doi: 10.1258/002367707779399446.

Abstract

Tail biopsies are routinely taken to genotype genetically modified mice. However, the effect of this procedure on the wellbeing of the animals has rarely been investigated. Thus, it has not yet been clearly demonstrated to what extent the mice suffer from tail biopsy (TB) and for how long. The aim of our study was to assess the impact of a single TB on the physiological and behavioural parameters of adult mice and to investigate whether or not anaesthesia can be beneficial. Body weight (BW) curves, daily food/water consumption and telemetric measurements of heart rate, body core temperature, and locomotor activity were recorded for three days following TB, both with and without anaesthesia with methoxyflurane (MOF) or diethylether (ether). Additionally, the impact of anaesthesia alone was characterized. TB without anaesthesia induced an increase in heart rate and locomotor activity for 1 h. Body core temperature was elevated for 2 h. In contrast, heart rate was increased for up to 4 h after anaesthesia. Body core temperature remained altered for up to 20 h after exposure to ether and for 44 h after exposure to MOF. BW was slightly reduced after MOF. Cases of death occurred exclusively under ether at a rate of 7%. Our results indicate a short-lived impact of a TB, whereas anaesthesia with either MOF or ether induced remarkable alterations in the parameters analysed. In conclusion, these types of anaesthesia did not improve mouse wellbeing following tail biopsy.

摘要

通常会采集尾部活检样本对基因编辑小鼠进行基因分型。然而,这一操作对动物健康的影响鲜有研究。因此,小鼠因尾部活检(TB)所受的影响程度以及持续时间尚未得到明确证实。我们研究的目的是评估单次TB对成年小鼠生理和行为参数的影响,并探究麻醉是否有益。在TB后的三天内,记录有无用甲氧氟烷(MOF)或乙醚麻醉情况下的体重(BW)曲线、每日食物/水消耗量以及心率、体核温度和运动活动的遥测数据。此外,还对单独麻醉的影响进行了表征。未麻醉的TB会使心率和运动活动在1小时内增加。体核温度升高2小时。相比之下,麻醉后心率增加长达4小时。接触乙醚后体核温度在长达20小时内保持变化,接触MOF后则在44小时内保持变化。MOF后BW略有下降。仅在使用乙醚的情况下出现死亡病例,死亡率为7%。我们的结果表明TB的影响是短暂的,而使用MOF或乙醚麻醉会导致所分析参数出现显著变化。总之,这些类型的麻醉并不能改善小鼠尾部活检后的健康状况。

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