Jacobsen Kirsten R, Kalliokoski Otto, Teilmann Anne C, Hau Jann, Abelson Klas Sp
Department of Experimental Medicine, University of Copenhagen and University Hospitals, Copenhagen, Denmark.
J Am Assoc Lab Anim Sci. 2012 Jan;51(1):69-75.
Recognition of pain and stress is a common challenge when working with laboratory mice. The aim of the current study was to identify noninvasive parameters to assess the severity and duration of possible pain and stress after vasectomy in BALB/c mice. Mice underwent isoflurane anesthesia with or without vasectomy. Body weight, food and water intake, and fecal corticosterone metabolites (FCM) were measured 3 d before and 3 d after the procedure. Behavior was recorded 1, 2, 4, and 8 h after the procedure. Food and water consumption and defecation were reduced postoperatively in the vasectomized group compared with mice given anesthesia only. FCM were elevated the first day after anesthesia in the control mice but not in the vasectomized group. Vasectomy resulted in behavioral changes that were not seen in the group that was anesthetized only. In conclusion, food and water consumption and pain-related behaviors, but not FCM, may be useful as noninvasive parameters to assess postoperative pain and stress in vasectomized mice.
在对实验小鼠进行研究时，识别疼痛和应激是一项常见的挑战。本研究的目的是确定非侵入性参数，以评估BALB/c小鼠输精管切除术后可能出现的疼痛和应激的严重程度及持续时间。小鼠接受异氟烷麻醉，部分进行输精管切除术。在手术前3天和手术后3天测量体重、食物和水摄入量以及粪便皮质酮代谢物（FCM）。在手术后1、2、4和8小时记录行为。与仅接受麻醉的小鼠相比，输精管切除组术后食物和水的消耗量以及排便量减少。对照组小鼠在麻醉后第一天FCM升高，但输精管切除组未升高。输精管切除术导致了行为变化，而仅接受麻醉的组未出现这种变化。总之，食物和水的消耗量以及与疼痛相关的行为，而非FCM，可能作为评估输精管切除术后小鼠疼痛和应激的非侵入性参数。
