Stipkovits L, Nicolet J, Haldimann A, Frey J
Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest.
Mol Cell Probes. 1991 Dec;5(6):451-7. doi: 10.1016/s0890-8508(05)80017-9.
Mycoplasma hyopneumoniae, the principal aetiological agent of porcine enzootic pneumonia, synthesizes a 36 kDa protein (P36) which is an early and strong immunogenic factor in experimentally and naturally infected swine. Polyclonal antibodies were made against the recombinant P36 protein in rabbits and used for the identification of M. hyopneumoniae by the immunoblot technique. The proteins from the M. hyopneumoniae reference strains and from 13 M. hyopneumoniae field strains isolated from naturally infected pigs in Switzerland, Hungary, France and Canada were analysed by the immunoblot technique using anti-P36 antibodies. All 13 field strains and the three reference J strains of M. hyopneumoniae, received from different collections and laboratories, exhibited a strong reaction with a protein of 36 kDa indicating that the P36 protein is a common M. hyopneumoniae antigen. None of the different porcine Mycoplasma species including M. flocculare, M. hyorhinis, M. hyosynoviae, A. axanthum, A. laidlawii and A. granularum showed any reaction on the immunoblot with the anti-P36 antibodies. In addition, we have found no reaction with anti-P36 antibodies using 47 different Mycoplasma or Acholeplasma species isolated from human, mice, rat, poultry, ruminant, dog and cat. In conclusion we have shown that P36 is a protein that is a common antigen of M. hyopneumoniae strains and is not found in other Mycoplasma or Acholeplasma species tested. Because of its high specificity, P36 protein, or antibodies made against this protein can be used for the identification of M. hyopneumoniae strains.
猪肺炎支原体是猪地方性肺炎的主要病原体，它能合成一种36 kDa的蛋白质（P36），在实验感染和自然感染的猪中，该蛋白是一种早期且强效的免疫原性因子。用重组P36蛋白在兔体内制备了多克隆抗体，并通过免疫印迹技术用于鉴定猪肺炎支原体。使用抗P36抗体，通过免疫印迹技术分析了来自猪肺炎支原体参考菌株以及从瑞士、匈牙利、法国和加拿大自然感染猪中分离出的13株猪肺炎支原体田间菌株的蛋白质。所有13株田间菌株以及从不同收藏机构和实验室获得的3株猪肺炎支原体参考J菌株，均与一种36 kDa的蛋白质发生强烈反应，表明P36蛋白是猪肺炎支原体的一种共同抗原。包括絮状支原体、猪鼻支原体、猪滑膜支原体、无色支原体、莱氏无胆甾原体和颗粒无胆甾原体在内的不同猪支原体种类，在免疫印迹中均未与抗P36抗体发生任何反应。此外，我们发现从人、小鼠、大鼠、家禽、反刍动物、狗和猫中分离出的47种不同支原体或无胆甾原体，与抗P36抗体均无反应。总之，我们已证明P36是猪肺炎支原体菌株的一种共同抗原，在其他测试的支原体或无胆甾原体种类中未发现。由于其高特异性，P36蛋白或针对该蛋白制备的抗体可用于鉴定猪肺炎支原体菌株。
