Young T F, Ross R F
Am J Vet Res. 1987 Apr;48(4):651-6.
An immunoblot procedure was used to evaluate porcine antibody response to inoculation with Mycoplasma hyopneumoniae. Mycoplasmas solubilized with sodium dodecyl sulfate were used as antigens. Antibodies to 5 antigens, estimated to be of molecular weight (mol wt) 110,000, 64,000, 50,000, 41,000, and 36,000, were detected in sera collected during the course of induced mycoplasmal pneumonia. Mycoplasma hyopneumoniae antigens, mol wt 110,000, 50,000, 41,000, and 36,000, cross-reacted with M flocculare when antigen prepared from M flocculare or hyperimmune serum against it were used in the immunoblot procedure. The 36,000-dalton (D) antigen reacted with M hyopneumoniae and M hyorhinis convalescent sera. The 64,000-D M hyopneumoniae antigen was the only antigen that did not cross-react with M flocculare or M hyorhinis. Exposure of immunoblot strips with antigens to trypsin before reacting them with the convalescent sera abolished binding ability of the 110,000-D and 36,000-D antigens, but had no effect on binding by 64,000-D, 50,000-D, or 41,000-D antigens. None of the 5 antigens bound to 11 lectins.
采用免疫印迹法评估猪对猪肺炎支原体接种的抗体反应。用十二烷基硫酸钠溶解的支原体用作抗原。在诱导性支原体肺炎病程中采集的血清中检测到针对5种抗原的抗体，估计其分子量分别为110,000、64,000、50,000、41,000和36,000。当用絮状支原体制备的抗原或其超免疫血清进行免疫印迹法检测时，猪肺炎支原体分子量为110,000、50,000、41,000和36,000的抗原与絮状支原体发生交叉反应。36,000道尔顿（D）的抗原与猪肺炎支原体和猪鼻支原体恢复期血清发生反应。64,000-D的猪肺炎支原体抗原是唯一不与絮状支原体或猪鼻支原体发生交叉反应的抗原。在用恢复期血清反应之前，将带有抗原的免疫印迹条带用胰蛋白酶处理，可消除110,000-D和36,000-D抗原的结合能力，但对64,000-D、50,000-D或41,000-D抗原的结合没有影响。这5种抗原均不与11种凝集素结合。
