Kaiser G, Micheel B
Department of Experimental and Clinical Immunology, Central Institute of Molecular Biology, Berlin-Buch, Germany.
Biomed Biochim Acta. 1991;50(8):1033-9.
A two-step immunoaffinity isolation procedure for human alpha-fetoprotein (AFP) was developed resulting in highly purified AFP at high yield. A monoclonal antibody immunoadsorbent was used in the first step. Elution of AFP was carried out at alkaline pH by a solution of 0.5 mol/l ammonia containing 0.5 mol/l sodium chloride. To remove impurities caused by the first step, an anti-mouse immunoglobulin antibody immunoadsorbent was applied in the second step.
开发了一种用于人甲胎蛋白（AFP）的两步免疫亲和分离程序，可高产量地获得高度纯化的AFP。第一步使用单克隆抗体免疫吸附剂。通过含有0.5 mol/l氯化钠的0.5 mol/l氨溶液在碱性pH下进行AFP的洗脱。为了去除第一步产生的杂质，第二步使用抗小鼠免疫球蛋白抗体免疫吸附剂。
