唐松草叶莨菪转化根中的托烷生物碱。

Tropane Alkaloids in Transformed Roots of Datura quercifolia.

机构信息

Department of Pharmacognosy, University of Geneva, Sciences II, 30, quai Ernest Ansermet, CH-1211 Geneva 4, Switzerland.

出版信息

Planta Med. 1994 Apr;60(2):158-62. doi: 10.1055/s-2006-959441.

DOI:10.1055/s-2006-959441

PMID:17236033

Abstract

Hairy root cultures of DATURA QUERCIFOLIA were established following infection with AGROBACTERIUM RHIZOGENES strain LBA 9402. Eight tropane alkaloids were identified in the hairy roots, hyoscyamine being the major constituent. The growth and the hyoscyamine content of transformed roots were investigated under various conditions. Gamborg B5, Murashige and Skoog, and Woody Plant media were tested. Gamborg B5 medium was the best for growth as well as for hyoscyamine accumulation. The influence of sucrose concentration was examined and a 5% concentration was found to be the most appropriate for growth and for alkaloid production. After 35 days of incubation in this medium, the hyoscyamine content of the roots was 1.24% based on dry weight. The influence of gibberellic acid and of Amberlite XAD-4 resin on hyoscyamine production was tested.

摘要

毛状根培养的 DATURA QUERCIFOLIA 被建立后感染 AGROBACTERIUM RHIZOGENES 菌株 LBA 9402。八种托烷生物碱在毛状根中被鉴定出来，其中莨菪碱是主要成分。在不同条件下研究了转化根的生长和莨菪碱含量。测试了 Gamborg B5、Murashige 和 Skoog 以及木本植物培养基。Gamborg B5 培养基最适合生长和莨菪碱积累。考察了蔗糖浓度的影响，发现 5%的浓度最适合生长和生物碱生产。在这种培养基中培养 35 天后，根中莨菪碱的含量为干重的 1.24%。测试了赤霉素和 Amberlite XAD-4 树脂对莨菪碱生产的影响。