Biswas Diptesh, Chakraborty Avijit, Mukherjee Swapna, Ghosh Biswajit
Plant Biotechnology Laboratory, Post Graduate Department of Botany, Ramakrishna Mission Vivekananda Centenary College, Kolkata, India.
Department of Microbiology, Dinabandhu Andrews College, Kolkata, India.
Front Plant Sci. 2023 Sep 4;14:1197555. doi: 10.3389/fpls.2023.1197555. eCollection 2023.
Secondary metabolites synthesized by the Solanaceous plants are of major therapeutic and pharmaceutical importance, many of which are commonly obtained from the roots of these plants. 'Hairy roots', mirroring the same phytochemical pattern of the corresponding root of the parent plant with higher growth rate and productivity, are therefore extensively studied as an effective alternative for the production of these metabolites. Hairy roots are the transformed roots, generated from the infection site of the wounded plants with With their fast growth, being free from pathogen and herbicide contamination, genetic stability, and autotrophic nature for plant hormones, hairy roots are considered as useful bioproduction systems for specialized metabolites. Lately, several elicitation methods have been employed to enhance the accumulation of these compounds in the hairy root cultures for both small and large-scale production. Nevertheless, in the latter case, the cultivation of hairy roots in bioreactors should still be optimized. Hairy roots can also be utilized for metabolic engineering of the regulatory genes in the metabolic pathways leading to enhanced production of metabolites. The present study summarizes the updated and modern biotechnological aspects for enhanced production of secondary metabolites in the hairy root cultures of the plants of Solanaceae and their respective importance.
茄科植物合成的次生代谢产物具有重要的治疗和药用价值，其中许多通常从这些植物的根部获取。“毛状根”反映了亲本植物相应根的相同植物化学模式，且生长速率和生产力更高，因此作为生产这些代谢产物的有效替代方法受到广泛研究。毛状根是受伤植物感染部位产生的转化根。由于其生长迅速、无病原体和除草剂污染、遗传稳定性以及对植物激素的自养特性，毛状根被认为是用于生产特殊代谢产物的有用生物生产系统。最近，已采用多种诱导方法来提高这些化合物在毛状根培养物中的积累量，以用于小规模和大规模生产。然而，在后一种情况下，仍需优化毛状根在生物反应器中的培养。毛状根还可用于对代谢途径中的调控基因进行代谢工程改造，以提高代谢产物的产量。本研究总结了茄科植物毛状根培养物中提高次生代谢产物产量的最新现代生物技术方面及其各自的重要性。
