Plant and Insect Biology Research Unit EA3900 BioPI-UPJV, University of Picardy Jules Verne, UFR Sciences, 33 rue Saint Leu, 80039 Amiens cedex, France.
Phytochem Anal. 2010 Jan-Feb;21(1):118-27. doi: 10.1002/pca.1180.
Hydroponics has been shown as a possible way to produce high quality plant biomass with improved phytochemical levels. Nevertheless, effects of plant biotic and abiotic environment can lead to drastic changes and plant growth conditions must be optimised.
To evaluate how much microbes and Agrobacterium rhizogenes TR7 wild strain may affect the tropane alkaloid profile in Datura innoxia Mill. plants cultivated in hydroponic conditions.
Datura innoxia Mill. plants were cultivated in hydroponic with sterile or non-sterile conditions. For half of the non-sterile plants, Agrobacterium rhizogenes TR7 strain was added to the nutrient solution for hydroponics. The tropane alkaloid content of leaves and roots was analysed by UFLC/ESI-HRMS and MS/MS. The metabolite profiles were compared using partial least square-discriminant analysis.
In sterile conditions, aerial parts contained more scopolamine than the roots. However, the diversity of tropane alkaloids was greater in roots. Furthermore, 21 known compounds and four non-elucidated tropane alkaloids were found. The tropane alkaloid profile was shown to be statistically different between sterile and non-sterile hydroponic conditions. The levels of 3-acetoxy-6-hydroxytropane and 3-hydroxylittorine were higher in plants inoculated with A. rhizogenes. Five other tropane compounds were found in higher amounts in non-axenic control plants. Hyoscyamine and scopolamine total contents were much higher in the whole plant co-cultivated with A. rhizogenes TR7 than in controls. Furthermore, the leaves and roots of axenic plants contained more alkaloids than non-sterile ones.
In hydroponic conditions, microbes induced variations of the phytochemical levels. Addition of A. rhizogenes TR7 into the nutrient solutions improved the total hyoscyamine and scopolamine production.
水培已被证明是一种生产高质量植物生物量并提高植物化学水平的可能方法。然而，植物生物和非生物环境的影响可能导致剧烈变化，必须优化植物生长条件。
评估在水培条件下，微生物和根癌农杆菌 TR7 野生株对 Datura innoxia Mill. 植物中托烷生物碱谱的影响。
Datura innoxia Mill. 植物在无菌或非无菌条件下进行水培。对于一半的非无菌植物，将根癌农杆菌 TR7 菌株添加到水培营养液中。采用 UFLC/ESI-HRMS 和 MS/MS 分析叶片和根系中的托烷生物碱含量。采用偏最小二乘判别分析比较代谢物谱。
在无菌条件下，地上部分的莨菪碱含量高于根部。然而，根部的托烷生物碱种类更多。此外，发现了 21 种已知化合物和 4 种未阐明的托烷生物碱。无菌和非无菌水培条件下的托烷生物碱谱存在统计学差异。用 A. rhizogenes 接种的植物中 3-乙酰氧基-6-羟基托烷和 3-羟基黎芦定的水平较高。在非无菌对照植物中发现了另外 5 种托烷化合物的含量较高。与对照相比，整株植物与 A. rhizogenes TR7 共培养的莨菪碱和东莨菪碱总含量要高得多。此外，无菌植物的叶片和根系比非无菌植物含有更多的生物碱。
在水培条件下，微生物引起了植物化学水平的变化。在营养液中添加 A. rhizogenes TR7 提高了总莨菪碱和东莨菪碱的产量。
