通过直接递送细胞内三磷酸腺苷促进皮肤伤口愈合。
Enhancing skin wound healing by direct delivery of intracellular adenosine triphosphate.
作者信息
Chiang Benjamin, Essick Eric, Ehringer William, Murphree Sidney, Hauck Mary Anne, Li Ming, Chien Sufan
机构信息
Department of Surgery, University of Louisville, Louisville, KY 40202, USA.
出版信息
Am J Surg. 2007 Feb;193(2):213-8. doi: 10.1016/j.amjsurg.2006.08.069.
Abstract
BACKGROUND
A new intracellular adenosine triphosphate (ATP) delivery technique has been developed and was tested for skin wound care.
METHODS
Eleven pairs of adult nude mice were used. ATP-vesicles were applied in 11 mice, and another 11 mice were treated with lipid vesicles only.
RESULTS
The group treated with ATP-encapsulated fusogenic small, unilamellar lipid vesicles healed faster than the group treated with only lipid vesicles. Histologic study indicated better developed granular tissue and re-epithelialization in the study group, and wound tissue vascular endothelial growth factor expressions were also higher in ATP-vesicles treated mice.
CONCLUSIONS
This intracellular ATP delivery system may provide a new hope for wound healing as well as the treatment of medical conditions in which ischemia is involved.
摘要
背景
已开发出一种新的细胞内三磷酸腺苷（ATP）递送技术，并对其进行了皮肤伤口护理测试。
方法
使用11对成年裸鼠。11只小鼠应用ATP囊泡，另外11只小鼠仅用脂质囊泡处理。
结果
用包裹ATP的融合性小单层脂质囊泡处理的组比仅用脂质囊泡处理的组愈合更快。组织学研究表明，研究组的肉芽组织发育更好，再上皮化更好，并且在ATP囊泡处理的小鼠中伤口组织血管内皮生长因子表达也更高。
结论
这种细胞内ATP递送系统可能为伤口愈合以及涉及缺血的医学病症的治疗提供新的希望。
相似文献
1
Enhancing skin wound healing by direct delivery of intracellular adenosine triphosphate.
Am J Surg. 2007 Feb;193(2):213-8. doi: 10.1016/j.amjsurg.2006.08.069.
2
Intracellular delivery of adenosine triphosphate enhanced healing process in full-thickness skin wounds in diabetic rabbits.
Am J Surg. 2010 Jun;199(6):823-32. doi: 10.1016/j.amjsurg.2009.05.040.
3
Intracellular adenosine triphosphate delivery enhanced skin wound healing in rabbits.
Ann Plast Surg. 2009 Feb;62(2):180-6. doi: 10.1097/SAP.0b013e31817fe47e.
4
Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
PLoS One. 2014 Mar 17;9(3):e91787. doi: 10.1371/journal.pone.0091787. eCollection 2014.
5
Intracellular ATP delivery using highly fusogenic liposomes.
Methods Mol Biol. 2010;605:377-91. doi: 10.1007/978-1-60327-360-2_26.
6
Topical vascular endothelial growth factor reverses delayed wound healing secondary to angiogenesis inhibitor administration.
Wound Repair Regen. 2005 Sep-Oct;13(5):506-12. doi: 10.1111/j.1067-1927.2005.00071.x.
7
Rapid tissue regeneration induced by intracellular ATP delivery-A preliminary mechanistic study.
PLoS One. 2017 Apr 5;12(4):e0174899. doi: 10.1371/journal.pone.0174899. eCollection 2017.
8
[Effects of reinforcing qi and nourishing blood recipes on vascular endothelial growth factor and microvessel count in granulation tissue in rats with chronic skin ulcers].
Zhong Xi Yi Jie He Xue Bao. 2008 Oct;6(10):1049-53. doi: 10.3736/jcim20081012.
9
Polydeoxyribonucleotide stimulates angiogenesis and wound healing in the genetically diabetic mouse.
Wound Repair Regen. 2008 Mar-Apr;16(2):208-17. doi: 10.1111/j.1524-475X.2008.00361.x.
10
Effects of a topical enamel matrix derivative on skin wound healing.
Wound Repair Regen. 2004 Jan-Feb;12(1):100-8. doi: 10.1111/j.1067-1927.2004.012117.x.
引用本文的文献
1
Therapeutic potential of human mesenchymal stromal cell-derived mitochondria in a rat model of surgical digestive fistula.
Sci Rep. 2025 Aug 9;15(1):29167. doi: 10.1038/s41598-025-13887-3.
2
Engineered ATP-Loaded Extracellular Vesicles Derived from Mesenchymal Stromal Cells: A Novel Strategy to Counteract Cell ATP Depletion in an In Vitro Model.
Int J Mol Sci. 2025 Apr 5;26(7):3424. doi: 10.3390/ijms26073424.
3
The Use of Extracellular Vesicles in Achilles Tendon Repair: A Systematic Review.
Biomedicines. 2024 Apr 23;12(5):942. doi: 10.3390/biomedicines12050942.
4
The Physiological Inorganic Polymers Biosilica and Polyphosphate as Key Drivers for Biomedical Materials in Regenerative Nanomedicine.
Int J Nanomedicine. 2024 Feb 8;19:1303-1337. doi: 10.2147/IJN.S446405. eCollection 2024.
5
Roles of extracellular adenosine triphosphate on the functions of periodontal ligament cells.
BDJ Open. 2023 Jul 8;9(1):28. doi: 10.1038/s41405-023-00147-7.
6
ENERGI-F703 gel, as a new topical treatment for diabetic foot and leg ulcers: A multicenter, randomized, double-blind, phase II trial.
EClinicalMedicine. 2022 Jul 10;51:101497. doi: 10.1016/j.eclinm.2022.101497. eCollection 2022 Sep.
7
Optimal Fluence and Duration of Low-Level Laser Therapy for Efficient Wound Healing in Mice.
Ann Dermatol. 2021 Aug;33(4):318-323. doi: 10.5021/ad.2021.33.4.318. Epub 2021 Jul 1.
8
Photobiomodulation by Near-Infrared 980-nm Wavelengths Regulates Pre-Osteoblast Proliferation and Viability through the PI3K/Akt/Bcl-2 Pathway.
Int J Mol Sci. 2021 Jul 15;22(14):7586. doi: 10.3390/ijms22147586.
9
Enhanced Skin Incisional Wound Healing With Intracellular ATP Delivery Macrophage Proliferation and Direct Collagen Production.
Front Pharmacol. 2021 Jun 16;12:594586. doi: 10.3389/fphar.2021.594586. eCollection 2021.
10
Intracellular ATP Delivery Causes Rapid Tissue Regeneration Upregulation of Cytokines, Chemokines, and Stem Cells.
Front Pharmacol. 2020 Jan 16;10:1502. doi: 10.3389/fphar.2019.01502. eCollection 2019.
本文引用的文献
1
Enhancement of wound healing by shikonin analogue 93/637 in normal and impaired healing.
Skin Pharmacol Physiol. 2004 Jan-Feb;17(1):49-56. doi: 10.1159/000074063.
2
Hyperbaric oxygen therapy of diabetic foot ulcers, transcutaneous oxymetry in clinical decision making.
Wound Repair Regen. 2003 Nov-Dec;11(6):458-61. doi: 10.1046/j.1524-475x.2003.11610.x.
3
Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts.
Lasers Surg Med. 2003;32(3):239-44. doi: 10.1002/lsm.10126.
4
Extracellular ATP is an autocrine/paracrine regulator of hypoxia-induced adventitial fibroblast growth. Signaling through extracellular signal-regulated kinase-1/2 and the Egr-1 transcription factor.
J Biol Chem. 2002 Nov 22;277(47):44638-50. doi: 10.1074/jbc.M203012200. Epub 2002 Sep 18.
5
Critiquing clinical research of new technologies for diabetic foot wound management.
J Foot Ankle Surg. 2002 Jul-Aug;41(4):251-9. doi: 10.1016/s1067-2516(02)80024-2.
6
Spontaneous lipid vesicle fusion with electropermeabilized cells.
FEBS Lett. 2002 May 8;518(1-3):135-8. doi: 10.1016/s0014-5793(02)02676-5.
7
The effect of cell division on the cellular dynamics of microinjected DNA and dextran.
Mol Ther. 2002 May;5(5 Pt 1):579-88. doi: 10.1006/mthe.2002.0581.
8
Science, medicine and the future: healing chronic wounds.
BMJ. 2002 Jan 19;324(7330):160-3. doi: 10.1136/bmj.324.7330.160.
9
Collagen fibril network and elastic system remodeling in a reconstructed skin transplanted on nude mice.
Matrix Biol. 2001 Nov;20(7):463-73. doi: 10.1016/s0945-053x(01)00162-7.
10
Use of a cell-based interactive wound dressing to enhance healing of excisional wounds in nude mice.
Wound Repair Regen. 2001 Jul-Aug;9(4):297-304. doi: 10.1046/j.1524-475x.2001.00297.x.
Zotero 插件