Cai Xuezhong, Cullen Bryan R
Center for Virology and Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
RNA. 2007 Mar;13(3):313-6. doi: 10.1261/rna.351707. Epub 2007 Jan 19.
Although H19 was the first imprinted noncoding transcript to be identified, the function of this conserved RNA has remained unclear. Here, we identify a 23-nucleotide microRNA derived from H19 that is endogenously expressed in human keratinocytes and neonatal mice and overexpressed in cells transfected with human or mouse H19 expression plasmids. These data demonstrate that H19 can function as a primary microRNA precursor and suggest that H19 expression results in the post-transcriptional downregulation of specific mRNAs during vertebrate development.
尽管H19是第一个被鉴定的印记非编码转录本，但其保守RNA的功能仍不清楚。在此，我们鉴定出一种源自H19的23个核苷酸的微小RNA，其在人角质形成细胞和新生小鼠中内源性表达，并在转染人或小鼠H19表达质粒的细胞中过表达。这些数据表明H19可作为主要的微小RNA前体，并提示H19的表达导致脊椎动物发育过程中特定mRNA的转录后下调。
