Anil Kumar S, Abyaneh Majid Kazemian, Gosavi S W, Kulkarni Sulabha K, Pasricha Renu, Ahmad Absar, Khan M I
Biochemical Sciences Division, National Chemical Laboratory, Pune, India.
Biotechnol Lett. 2007 Mar;29(3):439-45. doi: 10.1007/s10529-006-9256-7. Epub 2007 Jan 20.
Synthesis of silver nanoparticles using alpha-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10-25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.
首次证明了在体外使用α-烟酰胺腺嘌呤二核苷酸磷酸（α-NADPH）依赖性硝酸还原酶和植物螯合肽合成银纳米颗粒。在硝酸还原酶存在的情况下，银离子被还原，导致形成直径为10 - 25纳米的稳定银水溶胶，并由封端肽稳定。通过X射线衍射、透射电子显微镜、X射线光电子能谱和紫外-可见吸收对纳米颗粒进行了表征。这些研究将有助于设计一种合理的酶促策略，用于合成不同化学成分、形状和尺寸的纳米材料及其分离。
