Prokopenko P G, Borisenko S A, Makarov O V, Tatarinov Iu S
Biull Eksp Biol Med. 1991 Aug;112(8):185-8.
Antisera were obtained by rabbits immunization with pooled extract from ovarian carcinoma and its metastases into the ome tum. Using standard test-system antigen, precipitating in agar, was identified in the tissue of 12 out of 24 primary ovarian carcinomas and in 8 out of 20 metastases. It was not revealed in adult healthy internal organs tissues and in fetal tissues, except embryonal large intestine, where it was determined in trace amounts in isolated samples. Using immunodiffusion method it wasn't determined in blood serum of healthy people and patients, pregnant women and neonates. Immunochemical identification using standard test-systems showed that this antigen is not identical with already known carcino-embryonic antigen, placental, reactive and onco-ovarian proteins. It presents acid-soluble alpha-2-globulin (ASAG-2) with MW 55 kD determined by gel-filtration and 55 kD determined by electrophoresis in 10% polyacrylamide gel with dodecylsulfate under reducing conditions. Physico-chemical and antigenic properties of ASAG-2, as we think, give the opportunity to present it as a new onco-ovarian antigen, which differs from the known proteins.
通过用来自卵巢癌及其转移至网膜肿瘤的混合提取物免疫兔子来获得抗血清。使用标准检测系统，在24例原发性卵巢癌组织中的12例以及20例转移瘤组织中的8例中，鉴定出了在琼脂中沉淀的抗原。在成年健康内脏组织和胎儿组织中未发现该抗原，但在胚胎大肠中，在分离样本中检测到痕量该抗原。使用免疫扩散法，在健康人、患者、孕妇和新生儿的血清中未检测到该抗原。使用标准检测系统进行的免疫化学鉴定表明，该抗原与已知的癌胚抗原、胎盘蛋白、反应性蛋白和肿瘤卵巢蛋白不同。通过凝胶过滤测定其分子量为55kD，在还原条件下于10%十二烷基硫酸钠聚丙烯酰胺凝胶中电泳测定其分子量也为55kD，它呈现为酸溶性α-2-球蛋白（ASAG-2）。我们认为，ASAG-2的物理化学和抗原特性使其有机会被视为一种不同于已知蛋白质的新型肿瘤卵巢抗原。
