Falaleeva D M, Kalashnikov V V, Sanina I V, Baryshkov Iu A, Arion V Ia
Biull Eksp Biol Med. 1985 Feb;99(2):172-4.
A new interspecific human and animal thymic antigen (AgT-1) was identified immunochemically. It was shown that AgT-1 is a protein with a molecular mass about 40000 dalton, electrophoretic mobility of alpha 1-globulins and isoelectric points 4.0 and 4.5. Heating of the protein to 80 degrees C led to the loss of its immunochemical activity. Antisera to AgT-1 were obtained by immunization of rabbits by conjugated extract of bovine thymus in complete Freund's adjuvant. AgT-1 was identified immunochemically in bovine embryonal thymus, spleen and liver. In addition to these organs, AgT-1 was discovered in lung extracts of adult animals. Identical antigen was identified in the embryonal thymus and extracts of human small intestine. AgT-1 antibodies inhibited the biological activity of the active thymic fraction (AFT-6) in recovering the sensitivity of spleen fRFC from thymectomized mice to the inhibitory action of azathioprin. The data indicate that the biological activity of AFT-6 is partly due to the molecules having antigenic determinants of AgT-1.
通过免疫化学方法鉴定出一种新的种间人类和动物胸腺抗原（AgT-1）。结果表明，AgT-1是一种蛋白质，分子量约为40000道尔顿，具有α1球蛋白的电泳迁移率，等电点为4.0和4.5。将该蛋白质加热至80℃会导致其免疫化学活性丧失。通过用牛胸腺结合提取物在完全弗氏佐剂中免疫兔子获得了抗AgT-1血清。在牛胚胎胸腺、脾脏和肝脏中通过免疫化学方法鉴定出AgT-1。除了这些器官外，在成年动物的肺提取物中也发现了AgT-1。在胚胎胸腺和人类小肠提取物中鉴定出相同的抗原。AgT-1抗体在恢复去胸腺小鼠脾脏fRFC对硫唑嘌呤抑制作用的敏感性方面，抑制了活性胸腺组分（AFT-6）的生物活性。数据表明，AFT-6的生物活性部分归因于具有AgT-1抗原决定簇的分子。
