Tachibana H, Kobayashi S, Nagakura K, Kaneda Y, Takeuchi T
Department of Parasitology, School of Medicine, Tokai University, Kanagawa, Japan.
J Protozool. 1991 Jul-Aug;38(4):329-34. doi: 10.1111/j.1550-7408.1991.tb01368.x.
Twenty monoclonal antibodies were produced against trophozoites of Entamoeba histolytica strains HK-9 and HM-1: IMSS. When reactivity to various enteric protozoa was examined by an indirect fluorescence antibody test, 15 of the monoclonal antibodies were strongly reactive with E. histolytica trophozoites. Species-specific antigens recognized by these monoclonal antibodies were located on the plasma membrane, nucleus, cytoplasm, and cytoskeletal structures of the trophozoites. Two of the remaining five monoclonals reacted strongly with trophozoites of the E. histolytica-like Laredo strain. The determinant antigen was located in the cytoplasm. The three remaining monoclonal antibodies were found to recognize cross-reactive antigens between E. histolytica and E. histolytica-like Laredo, E. hartmanni, E. coli, Dientamoeba fragilis, Giardia lamblia, and Trichomonas hominis. These three antibodies were also reactive with T. vaginalis and mammalian cells such as HeLa cells. Thus, the combined use of monoclonal antibodies seems capable of distinguishing E. histolytica and/or E. histolytica-like Laredo from other enteric protozoa.
针对溶组织内阿米巴菌株HK - 9和HM - 1:IMSS的滋养体产生了20种单克隆抗体。当通过间接荧光抗体试验检测对各种肠道原生动物的反应性时，15种单克隆抗体与溶组织内阿米巴滋养体强烈反应。这些单克隆抗体识别的种特异性抗原位于滋养体的质膜、细胞核、细胞质和细胞骨架结构上。其余5种单克隆抗体中有2种与类溶组织内阿米巴拉雷多菌株的滋养体强烈反应。决定性抗原位于细胞质中。发现其余3种单克隆抗体识别溶组织内阿米巴与类溶组织内阿米巴拉雷多、哈氏内阿米巴、结肠内阿米巴、脆弱双核阿米巴、蓝氏贾第鞭毛虫和人毛滴虫之间的交叉反应抗原。这三种抗体也与阴道毛滴虫和诸如HeLa细胞的哺乳动物细胞反应。因此，单克隆抗体的联合使用似乎能够将溶组织内阿米巴和/或类溶组织内阿米巴拉雷多与其他肠道原生动物区分开来。
