Klenk H D, Choppin P W
Proc Natl Acad Sci U S A. 1970 May;66(1):57-64. doi: 10.1073/pnas.66.1.57.
Glycosphingolipids of rhesus monkey kidney (MK), bovine kidney (MDBK), and two lines of hamster kidney (BHK21-F and Hak) cells have been compared with those of parainfluenza (SV5) virions grow in these cells. There are qualitative and quantitative differences in the neutral glycolipids and gangliosides found in the various cells. Cells with a high neutral glycolipid content (MK and MDBK) contain little or no gangliosides, and those with a relatively high ganglioside content (BHK21-F and HaK) contain little neutral glycolipid. Glycosphingolipids are found predominantly in the plasma membranes. Neutral glycolipids of the host cell membrane are incorporated into the envelope of SV5 virions, but neither gangliosides nor protein-bound neuraminic acid are found in virions. The absence of neuraminic acid from the virion may be due to the action of viral neuraminidase.
已将恒河猴肾（MK）、牛肾（MDBK）以及两株仓鼠肾细胞系（BHK21 - F和Hak）中的糖鞘脂与在这些细胞中生长的副流感病毒（SV5）病毒粒子的糖鞘脂进行了比较。在各种细胞中发现的中性糖脂和神经节苷脂存在质和量的差异。中性糖脂含量高的细胞（MK和MDBK）几乎不含或不含神经节苷脂，而神经节苷脂含量相对较高的细胞（BHK21 - F和HaK）几乎不含中性糖脂。糖鞘脂主要存在于质膜中。宿主细胞膜的中性糖脂被整合到SV5病毒粒子的包膜中，但在病毒粒子中未发现神经节苷脂和与蛋白质结合的神经氨酸。病毒粒子中缺乏神经氨酸可能是由于病毒神经氨酸酶的作用。
