Heldin C H, Usuki K, Miyazono K
Ludwig Institute for Cancer Research, Uppsala, Sweden.
J Cell Biochem. 1991 Nov;47(3):208-10. doi: 10.1002/jcb.240470304.
Platelet-derived endothelial cell growth factor (PD-ECGF) is a 45 kDa single chain polypeptide which stimulates endothelial cell growth and chemotaxis in vitro and angiogenesis in vivo. Analysis of a full length PD-ECGF cDNA revealed an open reading frame coding for 482 amino acids without homology to other known proteins. No signal sequence was observed, and analysis of the biosynthesis and processing of PD-ECGF in a thyroid carcinoma cell line revealed that PD-ECGF is released only very slowly. PD-ECGF becomes covalently associated with nucleotide triphosphates (e.g., ATP) in vivo, as well as in vitro. The physiological significance of this posttranslational modification remains to be elucidated. The tissue distribution and target cell specificity of PD-ECGF suggest roles in angiogenesis (e.g., during wound healing and in the developing placenta), as well as in the maintenance of the integrity of the endothelial cell lining of large vessels.
血小板衍生内皮细胞生长因子（PD - ECGF）是一种45千道尔顿的单链多肽，它在体外可刺激内皮细胞生长和趋化作用，在体内可促进血管生成。对全长PD - ECGF cDNA的分析揭示了一个编码482个氨基酸的开放阅读框，该阅读框与其他已知蛋白质无同源性。未观察到信号序列，并且对甲状腺癌细胞系中PD - ECGF的生物合成和加工分析表明，PD - ECGF释放非常缓慢。PD - ECGF在体内和体外都会与三磷酸核苷酸（如ATP）发生共价结合。这种翻译后修饰的生理意义仍有待阐明。PD - ECGF的组织分布和靶细胞特异性表明其在血管生成（如在伤口愈合和胎盘发育过程中）以及维持大血管内皮细胞内衬完整性方面发挥作用。
