Angelova P, Davidoff M, Kanchev L, Baleva-Ivanova K
Institute of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia.
Funct Dev Morphol. 1991;1(1):3-8.
The cellular localization of substance P immunoreactivity was demonstrated at light microscopy level in hamster gonads during foetal and postnatal development. Selective immunostaining was observed in both the foetal and the adult generation of Leydig cells, but only in the luteal and interstitial cells of the adult ovary. To date there have been few studies dealing with the potential role of substance P in the control of gonadal functions. To examine whether it exerts an effect on gonadal steroidogenesis, the gonads of newborn, prepuberal and adult hamsters were cultured in vitro in the presence of substance P (10(-7) M), HCG (5 IU/ml) or substance P + HCG. Production of the sex steroid hormones testosterone, oestradiol-17 beta and progesterone was measured by radioimmunoassay. Substance P was observed to have a modulatory effect on steroid production by foetal, immature and mature gonads of both sexes.
在胎儿期和出生后发育阶段的仓鼠性腺中，通过光学显微镜水平证实了P物质免疫反应性的细胞定位。在胎儿期和成年期的睾丸间质细胞中均观察到选择性免疫染色，但仅在成年卵巢的黄体细胞和间质细胞中观察到。迄今为止，关于P物质在性腺功能控制中的潜在作用的研究很少。为了研究它是否对性腺类固醇生成有影响，将新生、青春期前和成年仓鼠的性腺在含有P物质（10^(-7) M）、人绒毛膜促性腺激素（HCG，5 IU/ml）或P物质 + HCG的条件下进行体外培养。通过放射免疫测定法测定性类固醇激素睾酮、雌二醇-17β和孕酮的产生。观察到P物质对两性胎儿期、未成熟和成熟性腺的类固醇产生具有调节作用。
