Jayapal V, Sharmila K M, Selvibai G, Thyagarajan S P, Shanmugasundaram N, Subramanian S
Department of Microbiology, University of Madras, Pin Code, India.
Lepr Rev. 1991 Sep;62(3):310-4.
The ability of the fluorescein diacetate and ethidium bromide fluorescent staining method to assess the percentage of viable bacterial cells in suspension was compared with the plate counting method. Mycobacterium smegmatis and Escherichia coli bacterial cell suspensions were incubated at 60 degrees C. At different time intervals samples were taken and the percentage of viable cells in each sample was assessed by the fluorescent staining method and compared with the plate counting method. The fluorescent staining method showed a positive correlation with the plate counting method. However, the viable counts by the plate counting method were lower than the staining method when incubated at 60 degrees C, indicating a lag period in the decay of enzymes after bacterial death. Hence, the fluorescent staining technique can be used to assess the trend of bacterial death rather than to assess to exact number of viable bacilli.
将荧光素二乙酸酯和溴化乙锭荧光染色法评估悬浮液中活细菌细胞百分比的能力与平板计数法进行了比较。耻垢分枝杆菌和大肠杆菌细菌细胞悬浮液在60℃下孵育。在不同时间间隔取样，通过荧光染色法评估每个样品中活细胞的百分比，并与平板计数法进行比较。荧光染色法与平板计数法呈正相关。然而，在60℃孵育时，平板计数法的活菌计数低于染色法，这表明细菌死亡后酶的衰减存在滞后期。因此，荧光染色技术可用于评估细菌死亡趋势，而不是评估活芽孢杆菌的确切数量。
