Yoshioka K, Oh K B, Saito M, Nemoto Y, Matsuoka H
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Appl Microbiol Biotechnol. 1996 Nov;46(4):400-4. doi: 10.1007/BF00166236.
A new fluorescent derivative of D-glucose, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG), which had been previously developed for the analysis of glucose uptake activity by living cells, was investigated to evaluate its applicability for assaying the viability of yeast Candida albicans. Lineweaver-Burk plots showed to uptake of 2-NBDG to be competitively inhibited by D-glucose and not by L-glucose, which suggested the involvement of the glucose transporting system of C. albicans in the uptake of 2-NBDG. A good correlation was obtained between the yeast viability, determined by the plate-count method, and the 2-NBDG uptake activity of yeast cells (correlation constant: r = 0.97). This is expected to lead to the development of a new fluorescent probe for the determination of yeast cell viability.
一种新型的D-葡萄糖荧光衍生物，2-[N-(7-硝基苯并-2-恶唑-1,3-二氮杂环丁烷-4-基)氨基]-2-脱氧-D-葡萄糖(2-NBDG)，此前已被开发用于分析活细胞的葡萄糖摄取活性，本研究旨在评估其在检测白色念珠菌活力方面的适用性。Lineweaver-Burk图显示，2-NBDG的摄取受到D-葡萄糖的竞争性抑制，而不受L-葡萄糖的抑制，这表明白色念珠菌的葡萄糖转运系统参与了2-NBDG的摄取。通过平板计数法测定的酵母活力与酵母细胞的2-NBDG摄取活性之间具有良好的相关性(相关常数：r = 0.97)。这有望促成一种用于测定酵母细胞活力的新型荧光探针的开发。
