Program in Genetics and Cell Biology, Washington State University, Pullman, Washington 99164-4350.
Genetics. 1983 Apr;103(4):771-83. doi: 10.1093/genetics/103.4.771.
Ten enzyme loci were mapped in relation to their centromeres in gynogenetic diploid rainbow trout. Gene-centromere map distances, calculated under the assumption of complete interference, range from 1.1 cM for Ldh4 to 50 cM for Sod1. The Idh2 and Est1 loci are linked on the same chromosome arm.-The observation of close to 100% heterozygous gynogenetic diploids for the Sod1 and Mdh3,4 loci suggests that near-complete interference occurs on the chromosome arms carrying these loci. The high interference observed in this study and in several other species of fish may be related to the small size of fish chromosome arms.-Comparisons of map locations for the Ldh3 and Ldh4 and the Mdh3 and Mdh4 loci, which were duplicated by a tetraploid event in the evolution of salmonid fish, reveal that they are located at similar distances from their centromeres. Comparative mapping of loci duplicated longer ago shows more variation in map location.-The high proportion of heterozygotes for some loci after gynogenesis involving second polar body retention demonstrates that this is not a practical method for producing homozygous inbred lines in rainbow trout; treatments suppressing the first cell division are more promising for this purpose.
十个酶基因座被定位在与它们的着丝粒的关系在雌核发育二倍体虹鳟。基因-着丝粒的map 距离，计算下的假设完全干扰，范围从 1.1 cM 的 Ldh4 至 50 cM 的 Sod1。Idh2 和 Est1 基因座位于同一染色体臂上。-接近 100%异质的雌核发育二倍体 Sod1 和 Mdh3,4 基因座的观察表明，在携带这些基因座的染色体臂上几乎完全发生干扰。在这项研究中和在其他几种鱼类中观察到的高干扰可能与鱼类染色体臂的小尺寸有关。-比较 Ldh3 和 Ldh4 和 Mdh3 和 Mdh4 基因座的图谱位置，这些基因座在鲑鱼鱼类的进化中通过四倍体事件重复，表明它们位于离着丝粒相似的距离。比较更久远的重复基因座的图谱位置显示出更多的变化。-在涉及第二极体保留的雌核发育后，一些基因座的杂合子比例很高，这表明这不是在虹鳟中产生纯系近交系的实用方法；抑制第一次细胞分裂的处理方法更有希望达到这一目的。
