Young W P, Wheeler P A, Fields R D, Thorgaard G H
Department of Zoology, Washington State University, Pullman 99164-4236, USA.
J Hered. 1996 Jan-Feb;87(1):77-80. doi: 10.1093/oxfordjournals.jhered.a022960.
Homozygous and hybrid clonal lines of rainbow trout (Oncorhynchus mykiss) were confirmed to be isogenic using multilocus DNA fingerprinting. Homozygous clones were produced by androgenesis and gynogenesis using gametes from androgenetic male and female rainbow trout, respectively. Isogenic F1 hybrid lines were produced by crossing homozygous fish from different strains. One line of hybrid clones showed segregation for maternally inherited DNA fingerprint markers. The female from this cross, the only presumptive homozygous gynogenetic individual used in this study, was thought to have been produced by gynogenesis followed by blockage of the first cleavage division, but based on the DNA fingerprint analysis, apparently was derived by spontaneous polar body retention that maintained heterozygosity at some loci. Mutations at DNA fingerprint loci were not observed, indicating relative stability of fingerprint loci in the clonal lines. DNA fingerprinting appears to be a useful tool for identifying and genetically monitoring clonal lines of rainbow trout. Isogenic lines of rainbow trout will facilitate the production of saturated genetic maps for rainbow trout and enhance such endeavors as quantitative trait locus (QTL) analysis and loss of heterozygosity (LOH) studies in tumors.
利用多位点DNA指纹图谱技术，证实虹鳟（Oncorhynchus mykiss）的纯合和杂交克隆系为同基因系。纯合克隆分别通过雄核发育和雌核发育产生，所用配子分别来自雄核发育的雄性和雌性虹鳟。同基因F1杂交系通过将不同品系的纯合鱼杂交产生。其中一个杂交克隆系显示出母系遗传的DNA指纹标记发生分离。此次杂交中的雌性，即本研究中唯一假定的纯合雌核发育个体，被认为是通过雌核发育，随后第一次卵裂受阻产生的，但基于DNA指纹分析，显然是通过自发保留极体产生的，这在某些位点维持了杂合性。未观察到DNA指纹位点的突变，表明克隆系中指纹位点具有相对稳定性。DNA指纹图谱似乎是鉴定和遗传监测虹鳟克隆系的有用工具。虹鳟的同基因系将有助于构建虹鳟的饱和遗传图谱，并加强诸如数量性状位点（QTL）分析和肿瘤杂合性缺失（LOH）研究等工作。
