North Carolina State University, Raleigh, North Carolina 27650.
Genetics. 1983 Jun;104(2):365-79. doi: 10.1093/genetics/104.2.365.
Mitochondrial DNAs (mtDNAs) were isolated from 93 diverse races of maize from Latin America. DNAs were examined by agarose gel electrophoresis of undigested DNA and by BamHI and EcoRI cleavage fragment analysis. Eighteen races contained plasmid-like mtDNAs. One race contained the S-1 and S-2 molecules associated with the S cytoplasmic male-sterile, and 17 were found to have the R-1 and R-2 plasmid-like DNAs. BamHI digestion of mtDNAs generated ten distinct electrophoretograms, and Eco RI digestion produced eight different fragment patterns. Races were assigned to one of 18 groups according to EcoRI and BamHI fragment patterns and whether or not they contained plasmid-like DNAs. Eight races produced restriction patterns similar to one of the characterized cytoplasmic male-steriles C, T, or S. Races from Meso-America and some from South America with Meso-American affinities were separated from other South American races. South American races were placed in three general classes of related groups. There was considerable agreement among the groupings here and those based on morphological and cytological affinities.
从拉丁美洲的 93 个不同的玉米品种中分离出了线粒体 DNA（mtDNA）。通过琼脂糖凝胶电泳检测未经消化的 DNA，以及 BamHI 和 EcoRI 切割片段分析来检查 DNA。18 个品种含有质粒样 mtDNA。一个品种含有与 S 细胞质雄性不育相关的 S-1 和 S-2 分子，17 个品种被发现含有 R-1 和 R-2 质粒样 DNA。BamHI 消化 mtDNA 产生了十种不同的电泳图谱，而 EcoRI 消化产生了八种不同的片段模式。根据 EcoRI 和 BamHI 片段模式以及是否含有质粒样 DNA，将品种分为 18 组之一。8 个品种产生的限制图谱与其中一种特征细胞质雄性不育 C、T 或 S 相似。来自中美洲和一些与中美洲有亲缘关系的南美洲品种与其他南美洲品种分开。南美品种被分为三类相关群体。这里的分组与基于形态学和细胞学亲缘关系的分组非常一致。
