果蝇中的 OM 突变与转座元件的插入有关。
OM Mutations in DROSOPHILA ANANASSAE Are Linked to Insertions of a Transposable Element.
机构信息
Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
出版信息
Genetics. 1986 Sep;114(1):125-35. doi: 10.1093/genetics/114.1.125.
Abstract
It has been hypothesized that Om mutability in Drosophila ananassae (involving spontaneous mutation at 20 loci, resulting in semidominant, nonpleiotropic eye morphology defects) was due to insertion of a transposable element, tom. One particularly unstable X-linked Om allele produced several derivatives, one of which has a more extreme Om phenotype and was accompanied by a singed bristle mutant, sn( 9g). DNA probes from the sn locus of D. melanogaster were used to clone the homologous region of D. ananassae. Analysis of sn(9g) DNA detected a 6.5-kb insert. Genomic Southern blotting and in situ hybridization techniques showed that this insert is repetitive and dispersed. The existence of the tom element is supported by genetic mapping that established homology between the 6.5-kb sn(9g) insert and Om mutants at the four X-linked loci tested.
摘要
据推测，果蝇 ananassae 中的 Om 可变性（涉及 20 个基因座的自发突变，导致半显性、非多效性眼睛形态缺陷）是由于转座因子 tom 的插入所致。一个特别不稳定的 X 连锁 Om 等位基因产生了几个衍生物，其中一个具有更极端的 Om 表型，并伴有烧焦的刚毛突变体 sn(9g)。使用来自黑腹果蝇 sn 基因座的 DNA 探针来克隆 D. ananassae 的同源区。对 sn(9g)DNA 的分析检测到一个 6.5kb 的插入。基因组 Southern 印迹和原位杂交技术表明，该插入是重复和分散的。转座因子 tom 的存在得到遗传作图的支持，该作图在四个测试的 X 连锁基因座上建立了 6.5kb sn(9g)插入与 Om 突变体之间的同源性。
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本文引用的文献
1
Controlling elements and the gene.控制元件与基因
Cold Spring Harb Symp Quant Biol. 1956;21:197-216. doi: 10.1101/sqb.1956.021.01.017.
2
Immunological method for mapping genes on Drosophila polytene chromosomes.在果蝇多线染色体上绘制基因图谱的免疫学方法。
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4381-5. doi: 10.1073/pnas.79.14.4381.
3
Nucleocytoplasmic relations in a mutator-suppressor system of Drosophila ananassae.果蝇变株抑制系统中的核质关系。
Genetics. 1981 May;98(1):77-90. doi: 10.1093/genetics/98.1.77.
4
Cytogenetic analysis of recombination in males of Drosophila ananassae.果蝇阿纳萨西雄性重组的细胞遗传学分析。
Chromosoma. 1983;88(4):286-92. doi: 10.1007/BF00292905.
5
Lambda replacement vectors carrying polylinker sequences.携带多克隆位点序列的λ置换载体。
J Mol Biol. 1983 Nov 15;170(4):827-42. doi: 10.1016/s0022-2836(83)80190-9.
6
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.生物素标记多核苷酸的酶促合成：新型核酸亲和探针。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6633-7. doi: 10.1073/pnas.78.11.6633.
7
Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method.通过一种新颖通用的方法克隆黑腹果蝇白色基因座的DNA序列。
Cell. 1981 Sep;25(3):693-704. doi: 10.1016/0092-8674(81)90176-8.
8
Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.抗生物素蛋白-生物素-过氧化物酶复合物（ABC）在免疫过氧化物酶技术中的应用：ABC法与未标记抗体（PAP）法的比较。
J Histochem Cytochem. 1981 Apr;29(4):577-80. doi: 10.1177/29.4.6166661.
9
The mitotic, polytene, and meiotic chromosomes of Drosophila ananassae.拟暗果蝇的有丝分裂染色体、多线染色体和减数分裂染色体。
J Hered. 1975 Nov-Dec;66(6):353-61. doi: 10.1093/oxfordjournals.jhered.a108647.
10
Nucleic acid hybridization to the DNA of cytological preparations.核酸与细胞学制剂的DNA杂交。
Methods Cell Biol. 1975;10:1-16. doi: 10.1016/s0091-679x(08)60727-x.
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