Ochiai A, Emura M, Riebe-Imre M, Mohr U, Tahara E
Medizinische Hochschule Hannover, Institut für Experimentelle Pathologie, Germany.
Exp Pathol. 1991;43(3-4):141-8. doi: 10.1016/s0232-1513(11)80108-8.
Four monoclonal antibodies designated as AMK-6, AMK-10, AMK-16 and AMK-27, were raised against merokeratin prepared from sheep's wool, and their cross-reactivity to epithelial cytokeratins were examined using immunoblot analysis and immunohistochemistry. AMK-16 which was specific to merokeratin in immunoblot analysis, demonstrated specific immunohistochemical staining of wool fibers of the sheep's skin. The other 3 monoclonal antibodies (AMK-6, -10, and -27) showed cross-reactivity towards epithelial cytokeratins with several molecular weights when examined by immunoblot analysis. However, each immunohistochemical staining of the sheep's skin with these monoclonal antibodies appeared to be cell type-specific. These results indicate that merokeratin and certain epithelial cytokeratins share many antigenic epitopes in common, and these monoclonal antibodies against merokeratin from sheep wool will be useful for the analysis of wool-forming cell differentiation.
制备了四种针对从羊毛中提取的角蛋白原纤维的单克隆抗体，分别命名为AMK - 6、AMK - 10、AMK - 16和AMK - 27，并使用免疫印迹分析和免疫组织化学方法检测了它们与上皮细胞角蛋白的交叉反应性。在免疫印迹分析中对角蛋白原纤维具有特异性的AMK - 16，在绵羊皮肤的羊毛纤维中显示出特异性免疫组织化学染色。通过免疫印迹分析检测时，其他三种单克隆抗体（AMK - 6、- 10和- 27）对几种分子量的上皮细胞角蛋白表现出交叉反应性。然而，用这些单克隆抗体对绵羊皮肤进行的每次免疫组织化学染色似乎都是细胞类型特异性的。这些结果表明，角蛋白原纤维和某些上皮细胞角蛋白具有许多共同的抗原表位，并且这些针对绵羊羊毛角蛋白原纤维的单克隆抗体将有助于分析羊毛形成细胞的分化。
