Goldring J P Dean
Department of Biochemistry, University of KwaZulu-Natal, Scottsville, South Africa.
Methods Mol Biol. 2019;1855:31-39. doi: 10.1007/978-1-4939-8793-1_3.
Measuring the concentration of proteins is an essential part of enzyme analysis or serves to monitor protein yields and losses during protein isolation procedures. Decisions on the usefulness of any protein isolation procedure depend on knowing the concentration of proteins before and after a procedure. Protein concentration in solution is generally measured with spectrophotometry in the UV range or in the presence of dyes or copper interacting with the protein. This review describes absorbance at 280 nm, the Lowry, Bradford (Coomassie Blue), and Smith (bicinchoninic acid) assays for measuring protein and includes suggestions for optimizing each method.
测量蛋白质浓度是酶分析的重要组成部分，或者用于监测蛋白质分离过程中的产量和损失。任何蛋白质分离方法是否有用，取决于能否得知该方法前后蛋白质的浓度。溶液中的蛋白质浓度通常采用紫外分光光度法，或在有与蛋白质相互作用的染料或铜存在的情况下进行测量。本综述介绍了用于测量蛋白质的280nm吸光度、洛瑞法、考马斯亮蓝法（Bradford法）和二喹啉甲酸法（Smith法），并给出了优化每种方法的建议。
