Koj A, Rokita H, Kordula T, Kurdowska A, Travis J
Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.
Biomed Biochim Acta. 1991;50(4-6):421-5.
The effects of various cytokines on synthesis and secretion of albumin and some proteinase inhibitors belonging to the class of macroglobulins, serpins and cysteine proteinase inhibitors were studied in the primary cultures of rat and mouse hepatocytes and established human hepatoma cell line Hep G2. In all tested systems interleukin 6 depressed the synthesis of albumin and enhanced the synthesis of antichymotrypsin (or contrapsin) and alpha-1-proteinase inhibitor, while in the rat alpha-2-macroglobulin and T-kininogen (thiostatin) were the major acute phase reactants. Smaller and variable effects were observed with interleukin-1, tumour necrosis factor, interferon-gamma, transforming growth factor-beta and epidermal growth factor. Searching for the feed-back regulatory mechanism responsible for induced synthesis of proteinase inhibitors we found that cultured human lung fibroblasts exposed to human alpha-1-antichymotrypsin or antichymotrypsin-cathepsin G complexes produce significantly more interleukin 6 which stimulates Hep G2 cells to augmented synthesis of several acute phase proteins.
在大鼠和小鼠肝细胞原代培养物以及已建立的人肝癌细胞系Hep G2中，研究了各种细胞因子对白蛋白以及属于巨球蛋白、丝氨酸蛋白酶抑制剂和半胱氨酸蛋白酶抑制剂类别的一些蛋白酶抑制剂的合成和分泌的影响。在所有测试系统中，白细胞介素6抑制白蛋白的合成，并增强抗胰凝乳蛋白酶（或抗胰蛋白酶）和α-1蛋白酶抑制剂的合成，而在大鼠中，α-2巨球蛋白和T-激肽原（抑胰肽酶）是主要的急性期反应物。白细胞介素-1、肿瘤坏死因子、干扰素-γ、转化生长因子-β和表皮生长因子的作用较小且变化不定。在寻找负责诱导蛋白酶抑制剂合成的反馈调节机制时，我们发现，暴露于人类α-1抗胰凝乳蛋白酶或抗胰凝乳蛋白酶-组织蛋白酶G复合物的培养人肺成纤维细胞产生的白细胞介素6显著增多，后者刺激Hep G2细胞增加几种急性期蛋白的合成。
