Koj A, Kurdowska A, Magielska-Zero D, Rokita H, Sipe J D, Dayer J M, Demczuk S, Gauldie J
Biochem Int. 1987 Mar;14(3):553-60.
Albumin, fibrinogen, alpha 1-acid glycoprotein and cysteine proteinase inhibitor were determined by electroimmunoassay in the media of primary cultures of rat hepatocytes exposed to dialysed supernatants of rat, mouse and human macrophages or to recombinant human and murine interleukin 1 and tumour necrosis factor. Recombinant cytokines in the range of 1 to 1000 ng/ml caused only reduction of albumin synthesis and slight stimulation of alpha 1 acid glycoprotein production while crude preparations of macrophage cytokines elicited typical acute phase response. The results suggest that interleukin 1 or tumour necrosis factor are not likely the principal mediators responsible for the direct stimulation of normal rat hepatocytes to acute phase protein synthesis.
采用电免疫分析法，测定了大鼠肝细胞原代培养物培养基中的白蛋白、纤维蛋白原、α1-酸性糖蛋白和半胱氨酸蛋白酶抑制剂，这些培养物分别暴露于大鼠、小鼠和人巨噬细胞的透析上清液，或重组人及小鼠白细胞介素1和肿瘤坏死因子中。浓度范围为1至1000 ng/ml的重组细胞因子仅导致白蛋白合成减少及α1-酸性糖蛋白产生略有刺激，而巨噬细胞细胞因子的粗制品引发了典型的急性期反应。结果表明，白细胞介素1或肿瘤坏死因子不太可能是直接刺激正常大鼠肝细胞合成急性期蛋白的主要介质。
