Department of Cellular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Genetics. 1981 Jul;98(3):505-27. doi: 10.1093/genetics/98.3.505.
This report describes a genetic study of salivary map region 39DE of the Drosophila genome, which is known to include the histone gene sequences (Pardue 1975; Liftonet al. 1977). Small deficiences extending proximally into 39DE were constructed by the segmental aneuploid method of Lindsleyet al. (1972). The translocational deficiencies obtained in this manner were gamma-irradiated to remove the Y translocational arms. One of these newly reconstituted deficiencies was then used to screen 10,000 gamma-irradiated second chromosomes for lethal mutations. The 32 lethals recovered from the screen were tested against several deficiencies and markers, crossed inter se and categorized according to their genetic properties. From these data, a preliminary complementation map was constructed of salivary region 39A-39F. The salivary map positions of certain of the complementation groups suggest that the mutants in these groups may affect histone gene functions.
本报告描述了对果蝇基因组唾液腺图谱区域 39DE 的遗传研究，该区域已知包含组蛋白基因序列（Pardue 1975；Lifton 等人，1977）。通过 Lindsley 等人（1972）的节段性三体方法构建了向近端延伸至 39DE 的小缺失。以这种方式获得的易位缺失用 γ 射线照射以去除 Y 易位臂。然后，使用其中一个新重建的缺失来筛选 10,000 个 γ 射线照射的第二染色体，以寻找致死突变。从筛选中回收的 32 个致死突变体与几个缺失和标记进行了测试，相互杂交，并根据其遗传特性进行了分类。根据这些数据，构建了唾液腺区域 39A-39F 的初步互补图谱。某些互补群的唾液腺图谱位置表明，这些群中的突变体可能影响组蛋白基因功能。
