Dvorak V, Aytekin A M, Alten B, Skarupova S, Votypka J, Volf P
Department of Parasitology, Charles University, Vinicna 7, Prague, 128 44, Czech Republic.
J Vector Ecol. 2006 Dec;31(2):229-38. doi: 10.3376/1081-1710(2006)31[229:acotiv]2.0.co;2.
Phlebotomus sergenti populations from different areas of the Mediterranean basin are known to exhibit high intraspecific variability. Previous studies of ITS2 revealed the presence of two branches that may represent sibling species. To corroborate this finding by other tools, two colonies of P. sergenti originating from Turkey and Israel, each belonging to a different ITS2 branch, were compared by three different methods: geometric morphometric analysis of wing shape, RAPD (random amplified polymorphic DNA), and cross-mating study. For geometric morphometric analysis, two-dimensional Cartesian coordinates of 16 landmarks from the wings were digitized and analyzed. Significant shape differences were found between colonies but not between sexes within each colony. RAPD results formed two distinctive clades corresponding to the origin of the colony but also showed heterogenity among members of both colonies. In cross-mating studies, viable hybrid F1 and F2 progeny were obtained when both Turkish males/Israeli females and Israeli males/Turkish females were crossed. F1 progeny was included in RAPD analysis and these hybrids formed a distinctive clade with an intermediate position between the two parental clades. No significant differences were found in egg production of crossed sand flies. The cross-mating study showed that there is no reproductive barrier between P. sergenti from different geographical areas. On the other hand, RAPD and geometric morphometric analysis revealed a significant difference between colonies and confirmed the suitability of previous ITS2 analysis for discrimination among sand fly populations. Further development of molecular markers should resolve a possible existence of sibling species within Phlebotomus sergenti.
已知地中海盆地不同地区的塞氏白蛉种群表现出高度的种内变异性。此前对ITS2的研究揭示了两个分支的存在，这两个分支可能代表同胞物种。为了用其他工具证实这一发现，对分别来自土耳其和以色列的两个塞氏白蛉菌落进行了三种不同方法的比较：翅形的几何形态计量分析、随机扩增多态性DNA（RAPD）以及杂交研究。这两个菌落分别属于不同的ITS2分支。对于几何形态计量分析，对翅膀上16个地标点的二维笛卡尔坐标进行数字化处理并分析。发现菌落之间存在显著的形状差异，但每个菌落内的雌雄之间没有差异。RAPD结果形成了两个与菌落起源相对应的独特分支，但也显示出两个菌落成员之间的异质性。在杂交研究中，当土耳其雄性/以色列雌性以及以色列雄性/土耳其雌性杂交时，获得了可存活的杂种F1和F2后代。F1后代被纳入RAPD分析，这些杂种形成了一个独特的分支，处于两个亲本分支之间的中间位置。在杂交白蛉的产卵量方面未发现显著差异。杂交研究表明，来自不同地理区域的塞氏白蛉之间不存在生殖障碍。另一方面，RAPD和几何形态计量分析揭示了菌落之间的显著差异，并证实了先前ITS2分析在区分白蛉种群方面的适用性。分子标记的进一步发展应能解决塞氏白蛉内可能存在的同胞物种问题。
