Moin-Vaziri Vahideh, Depaquit Jérôme, Yaghoobi-Ershadi Mohammad-Reza, Oshaghi Mohammad-Ali, Derakhshandeh-Peykar Pupak, Ferté Hubert, Kaltenbach Matthieu, Bargues Maria Dolores, Léger Nicole, Nadim Aboulhassan
Department of Medical Entomology and Vector Control, School of Public Health and Institute of Public Health Research, Medical Sciences/University of Tehran, P.O. Box 6446-14155, Tehran, Iran.
Acta Trop. 2007 Apr;102(1):29-37. doi: 10.1016/j.actatropica.2007.02.012. Epub 2007 Feb 27.
An intraspecific study on the morphological and molecular characteristics of Phlebotomus sergenti s.l., the main vector of Leishmania tropica, was performed on 28 Iranian populations from 11 provinces and a few samples from Greece, Morocco, Lebanon, Turkey, Pakistan, and Syria. Three morphotypes were identified as A, B and C, with some intermediate forms in the samples under investigation. Based on the number of setae and the width of basal lobe of coxite, differences between A and B morphotypes were highly significant. Excluding one unusual haplotype, sequence analysis of approximately 439 bp of mtDNA (a fragment of cytochrome B gene, tRNA for serine gene, and a fragment of NADH1 gene) revealed a 6-7% genetic distance within the Iranian populations and among the specimens of other countries. Neighbor-Joining (NJ) analysis confirmed the existence of three main groups within our samples. Although there was no consistency between morphotypes and genotypes, but an interrelationship was found between morphometry and morphotypes. Morphotype A, which was considered as P. sergenti sergenti, was the most prevalent in collection sites. Morphotype B, which was identified as Phlebotomus sergenti similis, is the first record of this subspecies in Iran, and was found to be sympatric with other morphotypes. Morphotype C had an elongated style in comparison with P. sergenti sergenti. Molecular database showed three main genetic structures. This is the first combined morphological and molecular studies on P. sergenti s.l. in Iran.
对热带利什曼原虫的主要传播媒介—— Sergenti 白蛉复合体（Phlebotomus sergenti s.l.）进行了种内形态学和分子特征研究，研究对象包括来自伊朗11个省份的28个种群以及来自希腊、摩洛哥、黎巴嫩、土耳其、巴基斯坦和叙利亚的一些样本。鉴定出三种形态型，分别为A、B和C，在所研究的样本中还有一些中间形态。根据刚毛数量和抱器基叶宽度，A和B形态型之间的差异非常显著。排除一个异常单倍型后，对约含439 bp的线粒体DNA（细胞色素B基因片段、丝氨酸转运RNA基因和NADH1基因片段）进行序列分析，结果显示伊朗种群内部以及其他国家标本之间的遗传距离为6 - 7%。邻接法（NJ）分析证实我们的样本中存在三个主要类群。虽然形态型和基因型之间没有一致性，但在形态测量与形态型之间发现了一种相互关系。被认为是 Sergenti 白蛉指名亚种（P. sergenti sergenti）的形态型A在采集地点最为常见。被鉴定为似 Sergenti 白蛉（Phlebotomus sergenti similis）的形态型B是该亚种在伊朗的首次记录，并且发现它与其他形态型同域分布。与 Sergenti 白蛉指名亚种相比，形态型C的阳茎较长。分子数据库显示出三种主要的遗传结构。这是伊朗首次对白蛉复合体进行形态学和分子学相结合的研究。
