Moin-Vaziri Vahideh, Oshaghi Mohammad Ali, Yaghoobi-Ershadi Mohammad Reza, Derakhshandeh-Peykar Pupak, Abaei Mohammad Reza, Mohtarami Fatemeh, Zahraei-Ramezani Ali Reza, Nadim Aboulhassan
Department of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Entomology and Vector Control, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran.
J Arthropod Borne Dis. 2016 Oct 4;10(4):462-473. eCollection 2016 Dec.
s.l. is considered the most likely vector of in Iran. Although two morphotypes- (A) and (B)-have been formally described, further morphological and a molecular analysis of mitochondrial cytochrome oxidase I (mtDNA-COI) gene revealed inconsistencies and suggests that the variation between the morphotypes is intraspecific and the morphotypes might be identical species.
We examined the sequence of the ITS2-rDNA of Iranian specimens of s.l., comprising , , and intermediate morphotypes, together with available data in Genbank.
Sequence analysis showed 5.2% variation among s.l. morphotypes. Almost half of the variation was due to the number of an AT microsatellite repeats in the center of the spacer. Nine haplotypes were found in the species constructing three main lineages corresponding to the origin of the colonies located in southwest (SW), northeast (NE), and northwest-center-southeast (NCS). Lineages NCS and NE included both typical and and intermediate morphotypes.
Phylogenetic sequence analysis revealed that, except for one Iranian sample, which was close to the European samples, other Iranian haplotypes were associated with the northeastern Mediterranean populations including Turkey, Cyprus, Syria, and Pakistan. Similar to the sequences of mtDNA COI gene, ITS2 sequences could not resolve from and did not support the possible existence of sibling species or subspecies within s.l.
在伊朗，实蝇属被认为是最有可能的传播媒介。尽管已经正式描述了两种形态型——实蝇A和实蝇B，但对线粒体细胞色素氧化酶I（mtDNA - COI）基因进行的进一步形态学和分子分析发现了不一致之处，并表明形态型之间的差异是种内差异，这些形态型可能是同一物种。
我们研究了伊朗实蝇属标本的ITS2 - rDNA序列，包括实蝇A、实蝇B和中间形态型，同时参考了Genbank中的现有数据。
序列分析显示实蝇属形态型之间存在5.2%的差异。几乎一半的差异是由于间隔区中心AT微卫星重复序列的数量不同。在该物种中发现了9个单倍型，构建了三个主要谱系，分别对应位于西南部（SW）、东北部（NE）和西北 - 中心 - 东南部（NCS）的种群起源。谱系NCS和NE包括典型的实蝇A和实蝇B以及中间形态型。
系统发育序列分析表明，除了一个与欧洲样本接近的伊朗样本外，其他伊朗单倍型与包括土耳其、塞浦路斯、叙利亚和巴基斯坦在内的地中海东北部种群相关。与mtDNA COI基因序列相似，ITS2序列无法区分实蝇A和实蝇B，也不支持实蝇属内可能存在姐妹物种或亚种的观点。
