Ito Akihiko, Hagiyama Man, Oonuma Junko, Murakami Yoshinori, Yokozaki Hiroshi, Takaki Miyako
Division of Surgical Pathology, Kobe University Graduate School of Medicine, Hyogo 650-0017, Japan.
J Neuroimmunol. 2007 Mar;184(1-2):209-13. doi: 10.1016/j.jneuroim.2006.12.008. Epub 2007 Jan 23.
Spermatogenic immunoglobulin superfamily (SgIGSF) expressed on nerve and mast cells, binds homophilically between both in culture. In the steady-state mesentery of mice, the proportion of morphologically degranulating mast cells was approximately 20%, and it increased nearly two-fold when the mesenteric nerve root was stimulated electrically. In contrast, there was no significant increase detectable in the mesentery of MITF-mutants, from which bone marrow-derived cultured mast cells (BMMCs) lack SgIGSF. BMMCs from SgIGSF-knockout mice transplanted to the mesentery of mast cell-deficient W/W(v) mice did not degranulate in response to the mesenteric nerve stimulation, whereas transfection with SgIGSF cDNA restored those responses. SgIGSF appeared to promote communication between nerves and mast cells in the murine mesentery.
在神经细胞和肥大细胞上表达的生精免疫球蛋白超家族（SgIGSF），在培养中两者之间能进行同源性结合。在小鼠的稳态肠系膜中，形态学上正在脱颗粒的肥大细胞比例约为20%，当电刺激肠系膜神经根时，这一比例几乎增加了两倍。相比之下，在小眼畸形相关转录因子（MITF）突变体的肠系膜中未检测到显著增加，其骨髓来源的培养肥大细胞（BMMCs）缺乏SgIGSF。将来自SgIGSF基因敲除小鼠的BMMCs移植到肥大细胞缺陷的W/W(v)小鼠的肠系膜中，对肠系膜神经刺激无脱颗粒反应，而用SgIGSF cDNA转染可恢复这些反应。SgIGSF似乎促进了小鼠肠系膜中神经细胞与肥大细胞之间的通讯。
