Wattiau Pierre, Van Hessche Mieke, Neubauer Heinrich, Zachariah Reena, Wernery Ulrich, Imberechts Hein
Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium.
J Clin Microbiol. 2007 Mar;45(3):1045-8. doi: 10.1128/JCM.02350-06. Epub 2007 Jan 24.
Close relatedness and genomic plasticity characterizing the high-threat pathogens Burkholderia pseudomallei and Burkholderia mallei render the molecular diagnosis of these species hard to guarantee with a maximal confidence level. This article describes fast molecular assays derived from compiled sequences of housekeeping genes determined in more than 1,000 strains. The assays proved to be robust and appropriate for general detection as well as species identification purposes.
密切的亲缘关系和基因组可塑性是高威胁病原体伯克霍尔德菌属的特征,这使得对这些菌种的分子诊断难以保证达到最高置信水平。本文描述了基于对1000多个菌株中确定的管家基因序列汇编得出的快速分子检测方法。这些检测方法被证明是可靠的,适用于一般检测以及菌种鉴定。