Translational Genomics Research Institute, Flagstaff, Arizona, United States of America.
PLoS One. 2010 Nov 12;5(11):e15413. doi: 10.1371/journal.pone.0015413.
A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.
设计了一种实时 PCR 检测方法 BurkDiff，以针对在区分这两个物种的单核苷酸多态性中包含 SNP 的布氏杆菌属假单胞菌和马耳他布鲁氏菌的独特保守区域。通过对 469 株布氏杆菌属假单胞菌、49 株马耳他布鲁氏菌和 390 株临床相关非靶标种属的 BurkDiff 进行筛选，评估了其敏感性和特异性。与传统物种鉴定方法的结果一致性以及对非靶标种属的无交叉反应性表明，BurkDiff 是一种用于检测和区分布氏杆菌属假单胞菌和马耳他布鲁氏菌的可靠、高度有效的检测方法。
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