Schmoock Gernot, Elschner Mandy, Sprague Lisa D
Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit, Naumburger Str. 96a, DE-07743, Jena, Germany.
National and OIE Reference Laboratory for Glanders, Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit, Naumburger Str. 96a, DE-07743, Jena, Germany.
Acta Vet Scand. 2015 Mar 7;57(1):13. doi: 10.1186/s13028-015-0104-4.
A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers.
Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B. pseudomallei field strains, and 1 B. thailandensis field strain from different geographic regions were tested and correctly identified. Testing of 55 non-Burkholderia bacterial species revealed 100% specificity of the assay. The minimum detection limit was 1 pg DNA or 160 GE for B. mallei and 130 GE for B. pseudomallei, respectively.
This assay enables the clear distinction between B. mallei and B. pseudomallei/B. thailandensis.
编码鼻疽伯克霍尔德菌趋化MotB蛋白的鞭毛运动基因motB中的一个移码突变已被用于设计一种使用荧光标记引物的传统双链PCR检测方法。
用一组13株伯克霍尔德菌标准菌株测试了物种特异性。对来自不同地理区域的总共41株鼻疽伯克霍尔德菌现场菌株、36株类鼻疽伯克霍尔德菌现场菌株和1株泰国伯克霍尔德菌现场菌株进行了测试,并得到了正确鉴定。对55种非伯克霍尔德菌属细菌的测试显示该检测方法具有100%的特异性。鼻疽伯克霍尔德菌的最低检测限分别为1 pg DNA或160 GE,类鼻疽伯克霍尔德菌为130 GE。
该检测方法能够明确区分鼻疽伯克霍尔德菌与类鼻疽伯克霍尔德菌/泰国伯克霍尔德菌。