O'Connor M, De Stasio E A, Dahlberg A E
Section of Biochemistry, Brown University, Providence, RI 02912.
Biochimie. 1991 Dec;73(12):1493-500. doi: 10.1016/0300-9084(91)90183-2.
Strains containing a series of restrictive and non-restrictive mutations in ribosomal protein S12 have been transformed with plasmids carrying the rrnB operon with mutations at positions 1409 and 1491 in 16S rRNA. The effects of the double-mutant constructs have been measured by growth rate, paromomycin and streptomycin sensitivity, resistance and dependence. The results demonstrate a functional interaction between the 1409-1491 region of rRNA and ribosomal protein S12.
含有核糖体蛋白S12一系列限制性和非限制性突变的菌株已用携带rrnB操纵子的质粒进行转化，该rrnB操纵子在16S rRNA的第1409和1491位存在突变。通过生长速率、巴龙霉素和链霉素敏感性、抗性和依赖性来测定双突变构建体的作用。结果表明rRNA的1409 - 1491区域与核糖体蛋白S12之间存在功能相互作用。
