含羞草中的穗花杉双黄酮及其对COX-1/COX-2催化的前列腺素生物合成的影响。

Amentoflavone from Biophytum sensitivum and its effect on COX-1/COX-2 catalysed prostaglandin biosynthesis.

作者信息

Bucar F, Jachak S M, Noreem Y, Kartnig T, Perera P, Bohlin L, Schubert-Zsilavecz M

出版信息

Planta Med. 1998 May;64(4):373-4. doi: 10.1055/s-2006-957455.

DOI:10.1055/s-2006-957455

Abstract

Amentoflavone (I3', II8-biapigenin) was isolated from the roots of Biophytum sensitivum DC. (Oxalidaceae) and proved to be a selective inhibitor of cyclooxygenase (COX)-1 catalysed prostaglandin biosynthesis when tested in vitro with an IC (50) value of 12.4 microM (standard: indomethacin, IC (50) = 1.1 microM). Doses of up to 37 microM showed only a slight inhibition in the corresponding COX-2 assay. Quantification of amentoflavone was carried out by reversed phase HPLC in methanolic and aqueous extracts of the roots, stems and leaves. Highest amounts of amentoflavone were detected in methanolic extracts of roots and stems (0.26-0.35%), while considerably lower amounts were detected in the corresponding water extracts.

摘要

从感应草（酢浆草科）的根部分离得到了穗花杉双黄酮（I3'，II8 - 双芹菜素），并且经体外测试证明，它是一种环氧化酶（COX）-1催化的前列腺素生物合成的选择性抑制剂，其半数抑制浓度（IC50）值为12.4微摩尔（标准：吲哚美辛，IC50 = 1.1微摩尔）。在相应的COX - 2检测中，高达37微摩尔的剂量仅表现出轻微抑制作用。通过反相高效液相色谱法对根、茎和叶的甲醇提取物和水提取物中的穗花杉双黄酮进行定量分析。在根和茎的甲醇提取物中检测到的穗花杉双黄酮含量最高（0.26 - 0.35%），而在相应的水提取物中检测到的含量则低得多。