Kinoshita O, Kawano Y, Yoshimi H, Ashida T, Yoshida K, Akabane S, Kuramochi M, Omae T
Division of Hypertension and Nephrology, National Cardiovascular Center, Osaka, Japan.
J Cardiovasc Pharmacol. 1991;17 Suppl 7:S511-3. doi: 10.1097/00005344-199100177-00146.
To evaluate the significance of endogenous endothelin (ET) in the maintenance of blood pressure in hypertensive and normotensive conditions, we examined the acute and chronic effects of anti-ET-1 antibody in spontaneously hypertensive rats (SHRs) and Wistar-Kyoto (WKY) rats. Immunoglobulin (Ig) solution purified from anti-ET-1 rabbit serum was injected intravenously in conscious rats aged 12-14 weeks (SHR: n = 7, WKY: n = 7). In the control group (SHR: n = 5, WKY: n = 4), Ig prepared from nonimmune rabbit serum was administered. One week after the first injections of Ig, second intravenous injections were given. After the intravenous injections of ET-1 antibody, there were no significant changes in directly measured blood pressure (BP) for up to 60 min in both strains. On the first, third, and seventh days after the first administration of ET-1 antibody, as well as on the first, third, and seventh days after the second injection, there were no appreciable differences in BP between SHR treated with anti-ET-1 antibody and SHR with control Ig. No significant changes in BP were observed throughout the observation period in WKY groups. Plasma levels of immunoreactive ET-1 were similar in SHR and WKY. This study suggests that circulating ET may not play a significant role in the maintenance of hypertension in SHRs or in the regulation of blood pressure in WKY rats.
为评估内源性内皮素（ET）在高血压和正常血压状态下维持血压的意义，我们研究了抗ET-1抗体对自发性高血压大鼠（SHR）和Wistar-Kyoto（WKY）大鼠的急性和慢性影响。将从抗ET-1兔血清中纯化的免疫球蛋白（Ig）溶液静脉注射到12 - 14周龄的清醒大鼠体内（SHR：n = 7，WKY：n = 7）。在对照组（SHR：n = 5，WKY：n = 4）中，给予从非免疫兔血清制备的Ig。首次注射Ig一周后，进行第二次静脉注射。静脉注射ET-1抗体后，两种品系大鼠在长达60分钟内直接测量的血压（BP）均无显著变化。在首次给予ET-1抗体后的第1、3和7天，以及第二次注射后的第1、3和7天，用抗ET-1抗体处理的SHR与用对照Ig处理的SHR之间的血压无明显差异。在整个观察期内，WKY组的血压未见显著变化。SHR和WKY的血浆免疫反应性ET-1水平相似。本研究表明，循环ET可能在SHR高血压的维持或WKY大鼠血压的调节中不起重要作用。
