Schmitt H, Mohr H, Lambrecht B, Knüver-Hopf J
Zentralinstitut Springe, DRK-Blutspendedienst Niedersachsen, BRD.
Beitr Infusionsther. 1991;28:82-91.
A procedure for the virus inactivation of single donor plasma is described. Virus inactivation is achieved by the combination of 1 mumol of phenothiazin dye, e.g. methylene blue and toluidine blue, and visible light. Using this method VSV, HSV and HIV, as well as other viruses, were inactivated. It could be shown that virus inactivation caused only a minimal reduction in the biological activities of coagulation factors and inhibitors. Furthermore, there was no indication for either neo-antigens or IgE-antibodies. In tolerance studies on dogs it was demonstrated that virus-inactivated autologous plasma caused no toxic effects.
描述了一种用于单采血浆病毒灭活的方法。通过1微摩尔吩噻嗪染料（如亚甲蓝和甲苯胺蓝）与可见光结合实现病毒灭活。使用该方法，水疱性口炎病毒（VSV）、单纯疱疹病毒（HSV）、人类免疫缺陷病毒（HIV）以及其他病毒均被灭活。结果表明，病毒灭活仅使凝血因子和抑制剂的生物活性出现极小程度的降低。此外，未发现新抗原或IgE抗体的迹象。在犬类耐受性研究中证实，病毒灭活的自体血浆未产生毒性作用。
