Mohr H, Lambrecht B, Schmitt H
DRK-Blutspendedienst Niedersachsen, Springe.
Beitr Infusionsther. 1993;31:25-30.
Fresh plasma from single-blood donations is virus-inactivated by illuminating the plasma units in their respective plastic bags with visible light for 1 h in the presence of 1 microM of the photoactive dye methylene blue. For "soft" viruses, e.g. the togavirus Semliki Forest, 0.3 microM are sufficient to achieve complete inactivation within 5-10 min. The infectious titer is reduced by 5-6 log 10 steps. To achieve the same degree of reduction in infectivity for more resistant viruses, e.g. vesicular stomatitis virus or SV 40, the virus-containing plasma at a dye concentration of 1 microM has to be light-treated for 30-45 min. More time is required at lower dye concentrations. These findings determined the conditions chosen for photodynamic virus inactivation of fresh plasma from single-blood donations.
单采血液的新鲜血浆通过在1微摩尔光活性染料亚甲蓝存在下，用可见光照射各自塑料袋中的血浆单位1小时来进行病毒灭活。对于“温和”病毒，例如披膜病毒塞姆利基森林病毒，0.3微摩尔就足以在5 - 10分钟内实现完全灭活。感染滴度降低5 - 6个对数10级。为了使更具抗性的病毒，如水泡性口炎病毒或SV 40，达到相同程度的感染性降低，含病毒血浆在染料浓度为1微摩尔时必须进行30 - 45分钟的光处理。在较低染料浓度下需要更多时间。这些发现确定了单采血液新鲜血浆光动力病毒灭活所选择的条件。
