Andreotti Renato
Department of Animal Health, Embrapa Beef Cattle, C.P. 154, Campo Grande, MS 79002-970, Brazil.
Exp Parasitol. 2007 May;116(1):66-70. doi: 10.1016/j.exppara.2006.11.009. Epub 2007 Jan 25.
Cattle tick control remains a serious problem in cattle farms worldwide, due to the limited success achieved with chemicals. Although the use of vaccines for tick control may open possibilities for an integrated approach, the search for other protective antigens is still necessary to improve control. Boophilus microplus is a rich source of BmTIs, serine proteinase inhibitors that are present throughout the tick's lifecycle. The present paper reports a pen trial conducted to evaluate the performance of a synthetic BmTI N-terminal fragment as antigen against the tick in bovines. The trial was conducted with two groups of eight crossbred cattle under controlled infestation: one group was vaccinated with BmTI peptide using saponin as adjuvant and the other was kept as control. Challenge was performed with 15,000 larvae. The specific IgG response was measured by ELISA, with successful outcomes for vaccinated animals. Vaccination resulted in an 18.4% level of efficacy when compared with the control group.
由于化学药物控制牛蜱的成效有限，牛蜱控制仍是全球养牛场面临的一个严重问题。尽管使用疫苗控制蜱虫可能为综合防治方法带来可能性，但仍需寻找其他保护性抗原以改善防治效果。微小牛蜱是牛蜱胰蛋白酶抑制剂（BmTIs）的丰富来源，这些丝氨酸蛋白酶抑制剂存在于蜱虫的整个生命周期中。本文报道了一项围栏试验，旨在评估合成的BmTI N端片段作为牛抗蜱抗原的性能。试验在两组各八头杂交牛身上进行，控制蜱虫感染情况：一组用皂素作为佐剂接种BmTI肽，另一组作为对照。用15000只幼虫进行攻毒。通过酶联免疫吸附测定法（ELISA）测量特异性IgG反应，接种疫苗的动物取得了成功结果。与对照组相比，接种疫苗的效果为18.4%。
