Charlier Johannes, Camuset Philippe, Claerebout Edwin, Courtay Bruno, Vercruysse Jozef
Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Res Vet Sci. 2007 Oct;83(2):194-7. doi: 10.1016/j.rvsc.2006.12.005. Epub 2007 Jan 26.
The Ostertagia-specific antibody levels in milk were monitored in 2 dairy herds to investigate seasonal variations and the relationship between individual and bulk tank milk antibody levels. Bulk tank and individual milk samples from all lactating animals were collected over a 1-year period at weekly and monthly intervals, respectively. The Ostertagia-specific antibody levels were measured with an indirect ELISA and the test results were expressed as optical density ratios (ODR). A clear seasonal pattern that followed the expected intake of infectious larvae was observed in the individual and bulk tank milk antibody levels of both herds. Within each herd, there was a large variation in the individual ODRs. This variation remained large when the distribution of individual ODRs was plotted according to high and low bulk tank milk ODR categories. The results suggest that the effect of seasonal variations on cut-off levels that predict production responses after anthelmintic control, needs to be assessed.
在2个奶牛场监测了牛奶中奥斯特他线虫特异性抗体水平,以调查季节性变化以及个体牛奶和奶罐牛奶抗体水平之间的关系。在1年时间里,分别以每周和每月的间隔采集了所有泌乳动物的奶罐牛奶和个体牛奶样本。用间接ELISA法测定奥斯特他线虫特异性抗体水平,检测结果以光密度比(ODR)表示。在两个牛群的个体牛奶和奶罐牛奶抗体水平中均观察到了遵循预期感染性幼虫摄入量的明显季节性模式。在每个牛群中,个体ODR存在很大差异。当根据奶罐牛奶ODR的高低类别绘制个体ODR的分布时,这种差异仍然很大。结果表明,需要评估季节性变化对预测驱虫控制后生产反应的临界值水平的影响。