Zhang Xue-han, Zeng Zheng-pei, Li Han-zhong, Zhou Ya-ru, Zhang Jing, Tong An-li, Yan Zhao-li
Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2006 Dec;28(6):766-9.
To compare the mRNA expression of renin-angiotensin-aldosterone system in human subcutaneous and visceral adipose tissues.
Total RNA was extracted from 12 human subcutaneous adipose tissues, 12 perirenal adipose tissue and 9 periadrenal adipose tissues. The expressions of angiotensinogen ( AGT) , renin, angiotensin converting enzyme ( ACE) , angiotensin converting enzyme 2 (ACE2), angiotensin I1 receptor type 1 (AT1), angiotensin II receptor type 2 (AT2 ), CYP11 B2, and their internal reference glyceraldehyde phosphate (GAPDH) were studied by reverse transcription-polymerase chain reaction. The ratios of each target genes were used to evaluate the expression levels of AGT, renin, ACE, ACE2, AT1, AT2, and CYP11B2 in different adipose tissues.
The mRNA expressions of AGT, ACE, ACE2, AT1, and AT2 were detected in human subcutaneous, perirenal, and periadrenal adipose tissues. However, CYPI B2 mRNA expression was not found in these three adipose tissues. The mRNA expressions of renin was only detected in perirenal and periadrenal adipose tissues, which was significantly higher in perirenal adipose tissues than in periadrenal adipose tissues ( P < 0. 05 ). The mRNA expressions of ACE and ACE2 in perirenal adipose tissues were significantly higher than that in subcutaneous adipose tissues ( P < 0. 05). The mRNA expressions of ACE were significantly higher than that of ACE2 in subcutaneous, perirenal, and periadrenal adipose tissues (P <0. 05). The mRNA expressions of AT1 were significantly lower than that of AT2 in periadrenal adipose tissues (P < 0. 05).
Local renin-angiotensin system exists in the adipose tissues; however, aldosterone is not synthesized in the adipose tissues.
比较肾素 - 血管紧张素 - 醛固酮系统在人皮下和内脏脂肪组织中的mRNA表达。
从12个人皮下脂肪组织、12个肾周脂肪组织和9个肾上腺周围脂肪组织中提取总RNA。采用逆转录 - 聚合酶链反应研究血管紧张素原（AGT）、肾素、血管紧张素转换酶（ACE）、血管紧张素转换酶2（ACE2）、血管紧张素Ⅱ1型受体（AT1）、血管紧张素Ⅱ2型受体（AT2）、CYP11B2及其内参甘油醛 - 3 - 磷酸脱氢酶（GAPDH）的表达。用各目的基因比值评估不同脂肪组织中AGT、肾素、ACE、ACE2、AT1、AT2和CYP11B2的表达水平。
在人皮下、肾周和肾上腺周围脂肪组织中检测到AGT、ACE、ACE2、AT1和AT2的mRNA表达。然而，在这三种脂肪组织中未发现CYPI B2 mRNA表达。肾素的mRNA表达仅在肾周和肾上腺周围脂肪组织中检测到，肾周脂肪组织中的表达显著高于肾上腺周围脂肪组织（P < 0.05）。肾周脂肪组织中ACE和ACE2的mRNA表达显著高于皮下脂肪组织（P < 0.05）。皮下、肾周和肾上腺周围脂肪组织中ACE的mRNA表达显著高于ACE2（P < 0.05）。肾上腺周围脂肪组织中AT1的mRNA表达显著低于AT2（P < 0.05）。
脂肪组织中存在局部肾素 - 血管紧张素系统；然而，脂肪组织中不合成醛固酮。
