硫醇化金纳米颗粒在增强葡萄糖氧化酶活性方面的应用。
Application of thiolated gold nanoparticles for the enhancement of glucose oxidase activity.
作者信息
Pandey Pratibha, Singh Surinder P, Arya Sunil K, Gupta Vinay, Datta Monika, Singh Sukhvir, Malhotra Bansi D
机构信息
Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India.
出版信息
Langmuir. 2007 Mar 13;23(6):3333-7. doi: 10.1021/la062901c. Epub 2007 Jan 30.
Glucose oxidase (GOx) has been covalently immobilized onto chemically synthesized thiolated gold nanoparticles (5-8 nm) via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The lower value of the Michaelis-Menton constant obtained for the immobilized (3.74 mM) GOx compared with that for the free (5.85 mM) GOx suggests significant enhancement in the activity of GOx attached to thiolated gold nanoparticles. The covalently immobilized GOx thiolated nanoparticles exhibit a response time of 30 s, a shelf life of more than 6 months, and improved tolerance to both pH and temperature.
葡萄糖氧化酶（GOx）已通过N-乙基-N'-(3-二甲基氨基丙基)碳二亚胺（EDC）和N-羟基琥珀酰亚胺（NHS）共价固定在化学合成的硫醇化金纳米颗粒（5-8纳米）上。与游离的葡萄糖氧化酶（5.85 mM）相比，固定化的葡萄糖氧化酶（3.74 mM）所获得的米氏常数较低，这表明附着在硫醇化金纳米颗粒上的葡萄糖氧化酶活性显著增强。共价固定的硫醇化金纳米颗粒葡萄糖氧化酶表现出30秒的响应时间、超过6个月的保质期以及对pH和温度的耐受性提高。
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