State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing 211816, China.
School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 210009, China.
Molecules. 2023 Jan 31;28(3):1357. doi: 10.3390/molecules28031357.
Immobilizing enzymes with nanozymes to catalyze cascade reactions overcomes many of the shortcomings of biological enzymes in industrial manufacturing. In the study, glucose oxidases were covalently bound to FeS nanozymes as immobilization carriers while chitosan encapsulation increased the activity and stability of the immobilized enzymes. The immobilized enzymes exhibited a 10% greater increase in catalytic efficiency than the free enzymes while also being more stable and catalytically active in environments with an alkaline pH of 9.0 and a high temperature of 100 °C. Additionally, the FeS nanozyme-driven double-enzyme cascade reaction showed high glucose selectivity, even in the presence of lactose, dopamine, and uric acid, with a limit of detection (LOD) (S/N = 3) as low as 1.9 × 10 M. This research demonstrates that nanozymes may be employed as ideal carriers for biological enzymes and that the nanozymes can catalyze cascade reactions together with natural enzymes, offering new insights into interactions between natural and synthetic biosystems.
将纳米酶固定化酶以催化级联反应克服了生物酶在工业制造中的许多缺点。在研究中，葡萄糖氧化酶通过共价键结合到 FeS 纳米酶上作为固定化载体，而壳聚糖包封增加了固定化酶的活性和稳定性。固定化酶的催化效率比游离酶提高了 10%，同时在 pH 值为 9.0 的碱性环境和 100°C 的高温下也更稳定和具有催化活性。此外，FeS 纳米酶驱动的双酶级联反应对葡萄糖具有高选择性，即使在存在乳糖、多巴胺和尿酸的情况下，检测限（LOD）（S/N = 3）低至 1.9×10 M。这项研究表明，纳米酶可用作生物酶的理想载体，并且纳米酶可以与天然酶一起催化级联反应，为天然和合成生物系统之间的相互作用提供了新的见解。
