一种针对热诱导型68 kDa热休克蛋白的新型单克隆抗体。
A novel monoclonal antibody directed against the heat-inducible 68 kDa heat shock protein.
作者信息
Heine L, Drabent B, Benecke B J, Günther E
机构信息
Abteilung Immungenetik der Universität, Göttingen, FRG.
出版信息
Hybridoma. 1991 Dec;10(6):721-30. doi: 10.1089/hyb.1991.10.721.
A novel monoclonal rat IgM antibody (8F7) is described which detects the inducible 68 kDa heat shock protein (hsp68) in man, mouse, rat, pig and cattle. Hsp68 expression is analysed in various cell types and cell lines by immunoblot with antibody 8F7 and in parallel by autoradiography after metabolic labeling with [35S]methionine. The new antibody cross-reacts with proteins of 180 and 48 kDa, which are not heat shock-inducible and show a pattern of expression different than hsp68.
本文描述了一种新型单克隆大鼠IgM抗体（8F7），它可检测人、小鼠、大鼠、猪和牛体内的诱导型68 kDa热休克蛋白（hsp68）。通过用抗体8F7进行免疫印迹，以及在用[35S]甲硫氨酸进行代谢标记后通过放射自显影术，对各种细胞类型和细胞系中的hsp68表达进行了分析。这种新抗体与180 kDa和48 kDa的蛋白质发生交叉反应，这些蛋白质不是热休克诱导型的，并且显示出与hsp68不同的表达模式。
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