颞叶癫痫中的载脂蛋白E：一项病例对照研究。
Apolipoprotein E in temporal lobe epilepsy: a case-control study.
作者信息
Kumar Amit, Tripathi Manjari, Pandey Ravindra M, Ramakrishnan Lakshmy, Srinivas M, Luthra Kalpana
机构信息
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi-110029, India.
出版信息
Dis Markers. 2006;22(5-6):335-42. doi: 10.1155/2006/951632.
PURPOSE
To investigate the relationship of apolipoprotein E (apoE) genotype, plasma levels of apoE and lipids in temporal lobe epilepsy (TLE) patients in Asian Indians. Status of plasma levels of Apo E in epilepsy patients has not been reported till date.
METHODS
ApoE gene polymorphism was analyzed in 58 patients with temporal lobe epilepsy (TLE) and 57 age and sex approximated controls using Polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). Levels of plasma apoE and lipids were measured using ELISA and enzymatic kits respectively.
RESULTS
The distribution of ApoE genotype in epilepsy patients and controls was comparable. Higher levels of plasma ApoE were observed in TLE patients as compared to controls (p=0.0001). Individuals with plasma levels of apoE > 190 mg/L were at 20 times higher odds (95%CI=2.46-163.34, p=0.005), while those with levels of apoE between 150-190 mg/L were at 4.9 times higher odds (95% CI=1.85-13.9, p=0.001), to develop TLE.
CONCLUSIONS
We have observed for the first time, high levels of plasma apoE in epilepsy patients. The findings of this case-control study suggest that apolipoprotein E may play an important role in epilepsy.
目的
研究亚洲印度裔颞叶癫痫（TLE）患者载脂蛋白E（apoE）基因型、血浆apoE水平与血脂之间的关系。迄今为止，尚未有关于癫痫患者血浆apoE水平状况的报道。
方法
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，对58例颞叶癫痫患者和57例年龄及性别相近的对照者进行apoE基因多态性分析。分别使用酶联免疫吸附测定法（ELISA）和酶试剂盒检测血浆apoE和血脂水平。
结果
癫痫患者和对照者中ApoE基因型的分布具有可比性。与对照组相比，TLE患者血浆ApoE水平更高（p=0.0001）。血浆apoE水平>190 mg/L的个体患TLE的几率高20倍（95%CI=2.46-163.34，p=0.005），而血浆apoE水平在150-190 mg/L之间的个体患TLE的几率高4.9倍（95%CI=1.85-13.9，p=0.001）。
结论
我们首次观察到癫痫患者血浆apoE水平较高。这项病例对照研究的结果表明，载脂蛋白E可能在癫痫中起重要作用。
Zotero 插件