Greenstein R J, Ybanez M M, Zhang R L, Bauman W A
Department of Surgery, VAMC Bronx 10468.
Mech Ageing Dev. 1991 Dec 2;61(2):113-21. doi: 10.1016/0047-6374(91)90010-w.
Age related differential gene expression occurs in the neuro-enteral axis. Brain and gut organ weight, total RNA, total protein and three peptides were quantified in 4-, 10- and 37-week-old Sprague-Dawley rats. As animals aged, total RNA decreased in the brain (0.65 +/- 0.3-0.28 +/- 0.03 mg/g), but remained stable in the gut (2.6 +/- 0.3-2.9 +/- 0.4 mg/g). Total protein concentration rose in the duodenum (612 +/- 28-734 +/- 34 mg/g), while levels remained stable in the brain (641 +/- 54-666 +/- 34 mg/g). Three peptides were studied, cholecystokinin (CCK), VIP and secretin. With increasing age, significant changes were found only in CCK a true neural-enteral peptide. The concentration of smaller molecular forms of CCK decreased in the brain (248 +/- 18-188 +/- 21 pmol/g), while they remained stable in the duodenum (33 +/- 2-36 +/- 3 pmol/g). By contrast, the concentration of the larger forms of CCK were stable in the brain (36 +/- 3-40 +/- 4 pmol/g), but rose in the gut (89 +/- 14-134 +/- 17 pmol/g). These data indicate that as rats age there is preprogrammed differential control of gene expression between brain and intestine.
与年龄相关的差异基因表达发生在神经-肠轴。对4周龄、10周龄和37周龄的斯普拉格-道利大鼠的脑和肠道器官重量、总RNA、总蛋白及三种肽进行了定量分析。随着动物年龄增长，脑中的总RNA减少（从0.65±0.3毫克/克降至0.28±0.03毫克/克），但肠道中的总RNA保持稳定（2.6±0.3毫克/克至2.9±0.4毫克/克）。十二指肠中的总蛋白浓度升高（从612±28毫克/克升至734±34毫克/克），而脑中的总蛋白水平保持稳定（641±54毫克/克至666±34毫克/克）。研究了三种肽，即胆囊收缩素（CCK）、血管活性肠肽（VIP）和促胰液素。随着年龄的增加，仅在真正的神经-肠肽CCK中发现了显著变化。脑中较小分子形式的CCK浓度降低（从248±18皮摩尔/克降至188±21皮摩尔/克），而十二指肠中的该浓度保持稳定（33±2皮摩尔/克至36±3皮摩尔/克）。相比之下，较大形式的CCK浓度在脑中保持稳定（36±3皮摩尔/克至40±4皮摩尔/克），但在肠道中升高（从89±14皮摩尔/克升至134±17皮摩尔/克）。这些数据表明，随着大鼠年龄增长，脑和肠道之间存在预先编程的基因表达差异调控。
