抗副结核分枝杆菌主要膜蛋白单克隆抗体的制备与鉴定
Production and characterization of monoclonal antibodies against a major membrane protein of Mycobacterium avium subsp. paratuberculosis.
作者信息
Bannantine John P, Radosevich Thomas J, Stabel Judith R, Berger Sven, Griffin J Frank T, Paustian Michael L
机构信息
National Animal Disease Center, ARS-USDA, 2300 North Dayton Ave., Ames, IA 50010, USA.
出版信息
Clin Vaccine Immunol. 2007 Mar;14(3):312-7. doi: 10.1128/CVI.00353-06. Epub 2007 Jan 31.
Abstract
The Mycobacterium avium subsp. paratuberculosis 35-kDa major membrane protein (MMP) encoded by MAP2121c is an important membrane antigen recognized in cattle with Johne's disease. In this study, purified recombinant MMP was used to produce two stable monoclonal antibodies, termed 8G2 and 13E1, which were characterized by immunoblotting, epitope mapping, and immunofluorescence microscopy.
摘要
由MAP2121c编码的鸟分枝杆菌副结核亚种35 kDa主要膜蛋白（MMP）是在患有副结核病的牛中识别出的一种重要膜抗原。在本研究中，纯化的重组MMP被用于制备两种稳定的单克隆抗体，分别命名为8G2和13E1，并通过免疫印迹、表位作图和免疫荧光显微镜对其进行了表征。
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