Li Lingling, Munir Shirin, Bannantine John P, Sreevatsan Srinand, Kanjilal Sagarika, Kapur Vivek
Department of Microbiology, University of Minnesota, 1500 Gortner Ave., St. Paul, MN 55108, USA.
Clin Vaccine Immunol. 2007 Jan;14(1):102-5. doi: 10.1128/CVI.00138-06. Epub 2006 Nov 1.
Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease, a chronic granulomatous enteritis of ruminants and other species. Detection of infection in animals is hampered by the lack of sensitive and specific diagnostic assays. We describe here an approach that utilizes translationally active PCR fragments for the rapid in vitro transcription and translation of recombinant proteins for antigen discovery in M. avium subsp. paratuberculosis. The investigations showed that the MAP1272c protein selectively reacts with sera from Johne's disease-positive cattle and represents an antigen of potential utility in M. avium subsp. paratuberculosis immunodiagnostics.
副结核分枝杆菌是反刍动物和其他物种的慢性肉芽肿性肠炎——约内氏病的病原体。由于缺乏灵敏且特异的诊断检测方法,动物感染的检测受到阻碍。我们在此描述一种方法,该方法利用具有翻译活性的PCR片段进行重组蛋白的快速体外转录和翻译,以在副结核分枝杆菌中发现抗原。研究表明,MAP1272c蛋白能与约内氏病阳性牛的血清发生选择性反应,是副结核分枝杆菌免疫诊断中具有潜在用途的一种抗原。